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Tpsab1 (NM_031187) Mouse Tagged ORF Clone
CAT＃: MG203662
- TrueORF®
Tpsab1 (tGFP-tagged) - Mouse tryptase alpha/beta 1 (Tpsab1)
ORF Plasmid: DDK
"NM_031187" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Mcp-7; Mcp7; Mcpt7; MMCP-7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203662 representing NM_031187
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAAAGCTGCTGCTGCTCACGCTGCCCCTCCTGTCCAGCCTGGTGCTTGCAGCCCCCGGTCCAGCTA TGACACGAGAAGGCATTGTGGGGGGACAGGAGGCACATGGGAACAAGTGGCCCTGGCAGGTGAGCCTGCG TGCCAATGACACCTACTGGATGCATTTCTGCGGTGGCTCCCTCATCCACCCACAGTGGGTGCTCACTGCG GCACACTGTGTGGGACCGGATGTTGCTGACCCCAACAAGGTCAGAGTACAGCTCCGTAAGCAGTACCTCT ATTACCATGACCACCTGATGACTGTGAGCCAGATCATCACACACCCCGACTTCTACATCGTCCAGGATGG GGCAGACATTGCCCTGCTGAAACTCACAAACCCTGTGAACATTTCTGACTATGTCCACCCTGTCCCCCTA CCTCCTGCCTCAGAGACCTTCCCCTCAGGAACGTTGTGCTGGGTGACAGGCTGGGGTAACATCGACAATG GTGTAAACCTGCCGCCACCATTTCCTTTGAAGGAGGTGCAAGTTCCCATTATAGAAAACCACCTTTGTGA CTTGAAGTATCACAAAGGTCTCATCACAGGTGACAATGTCCACATTGTCCGAGATGACATGCTGTGTGCT GGGAATGAAGGACATGACTCCTGCCAGGGCGACTCCGGAGGACCTCTGGTCTGCAAGGTAGAAGACACCT GGCTGCAGGCAGGCGTGGTCAGCTGGGGTGAGGGCTGCGCACAGCCCAACAGGCCTGGCATCTACACCCG GGTCACCTATTACTTGGATTGGATCCACCGCTATGTCCCCAAGGACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203662 representing NM_031187
Red=Cloning site Green=Tags(s) MLKLLLLTLPLLSSLVLAAPGPAMTREGIVGGQEAHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTA AHCVGPDVADPNKVRVQLRKQYLYYHDHLMTVSQIITHPDFYIVQDGADIALLKLTNPVNISDYVHPVPL PPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCA GNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHRYVPKDF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031187 |
| ORF Size | 819 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031187.2, NP_112464.2 |
| RefSeq Size | 1193 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 100503895 |
| UniProt ID | Q02844 |
| Gene Summary | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207327 | Tpsab1 (untagged) - Mouse tryptase alpha/beta 1 (Tpsab1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR203662 | Tpsab1 (Myc-DDK-tagged) - Mouse tryptase alpha/beta 1 (Tpsab1), transcript variant 1 |
CNY 2,400.00 |
|
| MR203662L3 | Lenti ORF clone of Tpsab1 (Myc-DDK-tagged) - Mouse tryptase alpha/beta 1 (Tpsab1), transcript variant 1 |
CNY 4,750.00 |
|
| MR203662L4 | Lenti ORF clone of Tpsab1 (mGFP-tagged) - Mouse tryptase alpha/beta 1 (Tpsab1), transcript variant 1 |
CNY 4,750.00 |
