Tmem25 (BC060057) Mouse Tagged ORF Clone

CAT#: MG203767

  • TrueORF®

Tmem25 (tGFP-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102)

ORF Plasmid: DDK tGFP



  "BC060057" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,200.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Tmem25 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 0610039J01Rik; AI429491
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203767 representing BC060057
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATTGCCTCTAAGCCAAGCTACCCTCCGGCACACACTGCTGCTCCTTCCTGCCCTCTTAAGTTCAG
GTCAAGGGGAGCTGGCGCCGCAAATTGATGGTCAGACCTGGGCTGAGAGAGCGCTTAGGGAGAATGAGCA
CCATGCCTTCACCTGTCGGGTTGCAGGAGGTTCTGCCACTCCCCGATTAGCCTGGTACCTGGATGGACAG
CTACAGGAGGCCACCACCTCCCGACTGCTGAGTGTGGGTGGGGACGCCTTCTCTGGAGGAACCAGCACTT
TCACTGTTACTGCCCAGCGCTCCCAACATGAGCTTAACTGCTCCCTGCAGGACCCAGGGAGTGGCCGGCC
AGCCAATGCCTCTGTCATCCTCAATGTGCAATTCAAACCAGAGATTGCCCAGGTTGGAGCCAAATACCAG
GAAGCTCAGGGCCCAGGCCTTCTGGTTGTCCTGTTTGCCCTGGTGCGGGCCAACCCACCTGCCAATGTCA
CCTGGATTGACCAGGATGGGCCAGTGACTGTCAATGCCTCTGACTTCCTGGTGCTGGATGCTCAGAACTA
TCCCTGGCTCACCAACCACACTGTGCAGCTGCAGCTCCGGAGCTTGGCACACAACCTCTCGGTGGTGGCC
ACCAATGACGTGGGTGTCACTAGCGCTTCCCTTCCAGCTCCAGGGCTCCTGGCCACACGGATAGAAGTGC
CACTGCTGGGCATCGTTGTGGCTGGAGGACTTGCCTTGGGCACTCTAGTGGGGTTCAGTACCTTGGTGGC
CTGCCTGGTCTGCAGGAAAGAGAAGAAAACAAAAGGCCCCTCCCGGCGTCCTTCTCTGATATCTAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203767 representing BC060057
Red=Cloning site Green=Tags(s)

MELPLSQATLRHTLLLLPALLSSGQGELAPQIDGQTWAERALRENEHHAFTCRVAGGSATPRLAWYLDGQ
LQEATTSRLLSVGGDAFSGGTSTFTVTAQRSQHELNCSLQDPGSGRPANASVILNVQFKPEIAQVGAKYQ
EAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNASDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVA
TNDVGVTSASLPAPGLLATRIEVPLLGIVVAGGLALGTLVGFSTLVACLVCRKEKKTKGPSRRPSLISR

TRTRPLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060057
ORF Size 837 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060057, AAH60057
RefSeq Size 2714 bp
RefSeq ORF 839 bp
Locus ID 71687
Gene Summary In neurons, modulates the degradation of NMDA receptor GRIN2B subunit. Plays a role in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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