Ppap2a (BC061161) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Hic53, LPP1, mPAP, LPP-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203848 representing BC061161 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGATTTGCGTGTTGCTGGCTGCCATGCCTA TGACTATTTTAAAATTGGGCAAAGTATATCCATTTCAGAGGGGCTTTTTCTGTACTGACAACAGCGTGAA GTACCCGTACCATGACAGTACCATCCCGTCCCGTATACTCGCCATACTGGGGCTTGGCTTACCCATTTTC TCTATGAGTATTGGAGAATCTCTGTCTGTTTACTTTAATGTCTTGCATTCGAATTCCTTTGTCGGCAATC CCTACATAGCCACCATTTACAAAGCCGTCGGAGCCTTTTTGTTCGGAGTCTCAGCTAGTCAGTCCTTGAC TGACATCGCTAAGTATACTATAGGCAGTTTGCGGCCGCACTTCTTGGCTATCTGTAACCCAGACTGGTCA AAAATCAACTGCAGTGATGGCTATATTGAGGACTACATATGTCAAGGGAATGAAGAGAAAGTCAAGGAGG GCAGGTTGTCTTTCTACTCGGGACACTCTTCATTCTCTATGTACTGCATGCTGTTTGTCGCACTTTATCT TCAAGCCAGGATGAAGGGAGACTGGGCAAGACTCTTACGACCCATGCTCCAGTTTGGGCTCATTGCTTTT TCCATATATGTGGGCCTTTCTCGAGTGTCTGACTACAAACACCACTGGAGTGACGTCACAGTTGGACTCA TTCAGGGAGCTGCTATGGCTATACTGGTTGCTTTGTATGTATCCGATTTCTTCAAGGACACACATTCTTA CAAAGAGAGAAAGGAAGAGGATCCACACACGACTCTCCATGAAACCGCCAGTTCACGGAACTACTCAACC AATCACGAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203848 representing BC061161 Red=Cloning site Green=Tags(s) MFDKTRLPYVALDVICVLLAAMPMTILKLGKVYPFQRGFFCTDNSVKYPYHDSTIPSRILAILGLGLPIF SMSIGESLSVYFNVLHSNSFVGNPYIATIYKAVGAFLFGVSASQSLTDIAKYTIGSLRPHFLAICNPDWS KINCSDGYIEDYICQGNEEKVKEGRLSFYSGHSSFSMYCMLFVALYLQARMKGDWARLLRPMLQFGLIAF SIYVGLSRVSDYKHHWSDVTVGLIQGAAMAILVALYVSDFFKDTHSYKERKEEDPHTTLHETASSRNYST NHEP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061161
ORF Size	854 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061161, AAH61161
RefSeq Size	1415 bp
RefSeq ORF	854 bp
Locus ID	19012
Gene Summary	Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), diacyl glycerol pyrophosphate (DGPP), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > C-1-P > S-1-P.[UniProtKB/Swiss-Prot Function]

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