United States
Germany
Japan
United Kingdom
China
Sumf1 (NM_145937) Mouse Tagged ORF Clone
CAT＃: MG203864
- TrueORF®
Sumf1 (tGFP-tagged) - Mouse sulfatase modifying factor 1 (Sumf1)
ORF Plasmid: DDK
"NM_145937" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,090.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA543204; AI463102; AI851573; FGE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203864 representing NM_145937
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCATTCCTGCTGGAGTATTCACAATGGGCACTGATGATCCTCAGATCAGGCAGGATGGAGAAG CCCCTGCCAGGAGAGTCACTGTTGATGGCTTTTACATGGACGCCTATGAAGTCAGCAATGCGGATTTTGA GAAGTTTGTGAACTCGACTGGCTATTTGACAGAGGCTGAGAAGTTTGGAGACTCTTTCGTCTTTGAAGGC ATGTTGAGCGAGCAAGTGAAAACGCATATCCACCAGGCAGTTGCAGCTGCTCCATGGTGGTTGCCTGTCA AGGGAGCTAATTGGAGACACCCAGAGGGTCCGGACTCCAGTATTCTGCACAGGTCAAATCATCCGGTTCT CCATGTTTCCTGGAACGATGCTGTTGCCTACTGCACATGGGCGGGCAAGAGGTTGCCTACTGAGGCAGAG TGGGAATACAGCTGTAGAGGAGGCCTGCAGAACAGGCTTTTCCCCTGGGGCAACAAACTGCAGCCCAAAG GACAGCATTATGCCAACATCTGGCAGGGCAAGTTTCCTGTGAGCAACACTGGCGAGGATGGCTTCCAAGG AACTGCCCCCGTTGATGCCTTTCCTCCCAATGGCTATGGCTTATACAACATAGTGGGGAATGTGTGGGAG TGGACCTCAGACTGGTGGACTGTTCACCATTCTGTTGAGGAAACGTTCAACCCAAAGGGTCCCACTTCTG GGAAAGACCGAGTGAAGAAGGGTGGATCCTACATGTGCCATAAGTCCTATTGCTATAGGTACCGCTGTGC AGCTCGAAGCCAGAACACACCAGATAGCTCTGCATCCAACCTGGGATTCCGATGTGCAGCCGACCACCTG CCCACCGCAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203864 representing NM_145937
Red=Cloning site Green=Tags(s) MVPIPAGVFTMGTDDPQIRQDGEAPARRVTVDGFYMDAYEVSNADFEKFVNSTGYLTEAEKFGDSFVFEG MLSEQVKTHIHQAVAAAPWWLPVKGANWRHPEGPDSSILHRSNHPVLHVSWNDAVAYCTWAGKRLPTEAE WEYSCRGGLQNRLFPWGNKLQPKGQHYANIWQGKFPVSNTGEDGFQGTAPVDAFPPNGYGLYNIVGNVWE WTSDWWTVHHSVEETFNPKGPTSGKDRVKKGGSYMCHKSYCYRYRCAARSQNTPDSSASNLGFRCAADHL PTAD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145937 |
| ORF Size | 852 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145937.1, NP_666049.1 |
| RefSeq Size | 2641 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 58911 |
| UniProt ID | Q8R0F3 |
| Gene Summary | Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201686 | Sumf1 (untagged) - Mouse sulfatase modifying factor 1 (Sumf1), (10ug) |
CNY 2,400.00 |
|
| MR203864 | Sumf1 (Myc-DDK-tagged) - Mouse sulfatase modifying factor 1 (Sumf1) |
CNY 3,710.00 |
|
| MR203864L3 | Lenti ORF clone of Sumf1 (Myc-DDK-tagged) - Mouse sulfatase modifying factor 1 (Sumf1) |
CNY 5,610.00 |
|
| MR203864L4 | Lenti ORF clone of Sumf1 (mGFP-tagged) - Mouse sulfatase modifying factor 1 (Sumf1) |
CNY 5,610.00 |
