Pir (NM_027153) Mouse Tagged ORF Clone

CAT#: MG203965

  • TrueORF®

Pir (tGFP-tagged) - Mouse pirin (Pir)

ORF Plasmid: DDK tGFP



  "NM_027153" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2310042L19Rik; Pirnl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203965 representing NM_027153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCTCTAAAAAGGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCAGAGGGGGTAGGAGCACGTG
TGCGCAGAAGCATTGGCAGACCCGAGTTGAAAAATCTGGATCCATTTTTACTGTTTGATGAATTTAAAGG
AGGAAGGCCGGGAGGATTTCCTGATCATCCACACCGAGGATTTGAAACAGTATCTTATCTTCTGGAAGGG
GGCAGCATGGCCCATGAAGACTTTTGTGGACATGTTGGTAAAATGAACCCAGGAGACCTACAGTGGATGA
CTGCAGGACGAGGCATTCTGCATGCTGAGATGCCCTGCTCAGAGGAGCCAGCTCATGGCTTGCAGCTGTG
GGTGAATTTGAGACGCTCAGAAAAGATGGTGGCGCCTCAGTACCAGGAACTGAAAAGTGAAGAGATCCCG
AAACCCACCAAAGATGGTGTGACAGTTGCTGTCATTTCAGGAGAAGCCTTGGGAATAAAGTCGAAGGTTT
ACACTCGCACACCAACCTTATATCTGGACTTCAAGTTGGACCAAGGAGCAAAGCATTCCCAGCCTATTCC
TAAAGGGTGGACAAGCTTCATTTATACCATCTCTGGAGATGTATATATTGGGCCTGATGATGCTCAACAA
AAGATAGAACCACATCACACAGCAGTCCTTGGAGAAGGTGACGCTGTCCAGTTGGAAAATAAGGACCCCA
AAAGAAGCCATTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTGTCCAACATGGTCCATTTGT
GATGAATACCAATGAGGAAATTTCTCAGGCCATTCTTGATTTCAGAAATGCAAAAAATGGTTTTGAGGGG
GCCAGAACCTGGAAGTCAAAGATTGGAAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203965 representing NM_027153
Red=Cloning site Green=Tags(s)

MASSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEG
GSMAHEDFCGHVGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRRSEKMVAPQYQELKSEEIP
KPTKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDQGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQ
KIEPHHTAVLGEGDAVQLENKDPKRSHFVLIAGEPLREPVVQHGPFVMNTNEEISQAILDFRNAKNGFEG
ARTWKSKIGN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_027153
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_027153.2, NP_081429.1
RefSeq Size 1487 bp
RefSeq ORF 873 bp
Locus ID 69656
UniProt ID Q9D711
Gene Summary Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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