Gnmt (NM_010321) Mouse Tagged ORF Clone
CAT#: MG204030
- TrueORF®
Gnmt (tGFP-tagged) - Mouse glycine N-methyltransferase (Gnmt)
ORF Plasmid: DDK
"NM_010321" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204030 representing NM_010321
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACAGCGTGTACCGTACCCGCTCCCTGGGGGTGGCGGCCGAAGGGCTCCCGGACCAGTATGCAG ATGGGGAGGCCGCACGTGTGTGGCAGCTGTACATCGGGGACACCCGCAGCCGTACCGCAGAGTACAAGGC GTGGTTGCTTGGGCTGTTGCGCCAGCACGGGTGCCACAGGGTGCTGGACGTAGCCTGTGGCACAGGAGTG GACTCCATCATGCTGGTGGAAGAGGGCTTCAGCGTGATGAGCGTGGACGCCAGCGACAAGATGCTGAAAT ATGCGCTTAAGGAGCGCTGGAACCGGAGGAAAGAGCCATCCTTTGACAATTGGGTCATTGAAGAAGCCAA CTGGTTGACGCTGGACAAAGATGTGCTTTCAGGAGATGGCTTTGATGCTGTCATCTGCCTTGGGAACAGT TTTGCTCACTTGCCAGACTGCAAAGGTGACCAGAGCGAGCACCGGCTGGAACTAAAGAACATTGCAAGCA TGGTGCGGCCCGGGGGCCTGCTGGTGATCGACCACCGCAACTACGACTATATCCTCAGCACAGGCTGTGC GCCCCCGGGGAAGAACATCTACTATAAGAGTGACCTGACCAAGGACATTACGACGTCAGTACTGACAGTC AACAACAAAGCCCACATGGTAACCCTGGACTACACAGTGCAGGTGCCAGGCACTGGCAGAGATGGCTCTC CTGGCTTCAGTAAGTTCCGGCTCTCTTACTACCCACACTGTTTGGCGTCTTTCACGGAGTTGGTGCGAGC AGCCTTTGGGGGCAGGTGCCAGCACAGCGTCCTGGGTGACTTCAAGCCCTACAAGCCTGGCCAGGCCTAC GTTCCCTGCTACTTCATCCATGTGCTCAAGAAGACAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204030 representing NM_010321
Red=Cloning site Green=Tags(s) MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGV DSIMLVEEGFSVMSVDASDKMLKYALKERWNRRKEPSFDNWVIEEANWLTLDKDVLSGDGFDAVICLGNS FAHLPDCKGDQSEHRLELKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTV NNKAHMVTLDYTVQVPGTGRDGSPGFSKFRLSYYPHCLASFTELVRAAFGGRCQHSVLGDFKPYKPGQAY VPCYFIHVLKKTD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010321 |
ORF Size | 879 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010321.1, NP_034451.1 |
RefSeq Size | 1042 bp |
RefSeq ORF | 882 bp |
Locus ID | 14711 |
UniProt ID | Q9QXF8 |
Gene Summary | Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200819 | Gnmt (untagged) - Mouse glycine N-methyltransferase (Gnmt), (10ug) |
CNY 2,400.00 |
|
MR204030 | Gnmt (Myc-DDK-tagged) - Mouse glycine N-methyltransferase (Gnmt) |
CNY 2,400.00 |
|
MR204030L3 | Lenti ORF clone of Gnmt (Myc-DDK-tagged) - Mouse glycine N-methyltransferase (Gnmt) |
CNY 4,750.00 |
|
MR204030L4 | Lenti ORF clone of Gnmt (mGFP-tagged) - Mouse glycine N-methyltransferase (Gnmt) |
CNY 4,750.00 |