Mpst (NM_138670) Mouse Tagged ORF Clone
CAT#: MG204112
- TrueORF®
Mpst (tGFP-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst)
ORF Plasmid: DDK
"NM_138670" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Mst |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204112 representing NM_138670
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCCCGCAACTTTTCCGAGCTCTGGTGTCTGCGCAGTGGGTGGCGGAGGCTCTGAAGGCCCCGC GCTCCTCACAGCCGCTGAAGTTACTGGACGCGTCCTGGTACCTACCCAAGCTGGGCCGCGATGCACGACG CGAGTTTGAGGAGCGCCACATCCCGGGCGCCGCCTTCTTCGACATCGATCGTTGCAGTGACCACACGTCC CCCTACGACCACATGCTGCCTAACGCCACGCACTTTGCCGACTACGCGGGCAGCTTGGGCGTGAGCGCCG CCACGCACGTCGTGATCTACGACGGCAGTGACCAGGGCCTCTATTCCGCTCCGCGGGTCTGGTGGATGTT CCGCGCCTTCGGCCACCACTCTGTGTCATTGCTGGACGGCGGCTTCCGCCACTGGCTGAATCAGAACCTG CCAATCAGCTCCGGCAAAAGCCACTCGGAGCCCGCGGAGTTCAGCGCGCAGCTCGATCCCTCTTTCATCA AGACCCACGAGGACATCCTGGAGAACTTGGATGCCCGGCGCTTCCAGGTGGTGGACGCCCGCGCAGCTGG CCGTTTCCAAGGCACCCAGCCAGAACCCCGAGATGGCATCGAACCTGGCCACATCCCTGGCTCAGTAAAC ATCCCGTTCACTGAATTCCTGACCAACGAAGGCCTGGAGAAGAGCCCAGAGGAGATCAAACGCCTGTTTA AGGAGAAGAAGGTGGACCTGTCTAAGCCCTTGGTAGCCACGTGTGGCTCCGGTGTCACAGCCTGCCACGT GGTCCTGGGGGCCTTCCTCTGTGGCAAGTCAGACGTGCCTGTCTATGATGGCTCCTGGGTAGAGTGGTAC ATGCGTGCCCAGCCAGAGCATATCATCTCTGAGGGCCGAGGGAAGACCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204112 representing NM_138670
Red=Cloning site Green=Tags(s) MAAPQLFRALVSAQWVAEALKAPRSSQPLKLLDASWYLPKLGRDARREFEERHIPGAAFFDIDRCSDHTS PYDHMLPNATHFADYAGSLGVSAATHVVIYDGSDQGLYSAPRVWWMFRAFGHHSVSLLDGGFRHWLNQNL PISSGKSHSEPAEFSAQLDPSFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVN IPFTEFLTNEGLEKSPEEIKRLFKEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKSDVPVYDGSWVEWY MRAQPEHIISEGRGKTQ SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138670 |
ORF Size | 891 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138670.2, NP_619611.2 |
RefSeq Size | 1438 bp |
RefSeq ORF | 894 bp |
Locus ID | 246221 |
UniProt ID | Q99J99 |
Gene Summary | Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207936 | Mpst (untagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR204112 | Mpst (Myc-DDK-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,400.00 |
|
MR204112L3 | Lenti ORF clone of Mpst (Myc-DDK-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,750.00 |
|
MR204112L4 | Lenti ORF clone of Mpst (mGFP-tagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,750.00 |