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Clk4 (BC002220) Mouse Tagged ORF Clone
CAT＃: MG204207
- TrueORF®
Clk4 (tGFP-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616)
ORF Plasmid: DDK
"BC002220" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI987988; C85119 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204207 representing BC002220
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCTTACATGTAGCAGTGAAAATTGTAAAAAATGTAGGCCGTTACCGGGAGGCAGCTCGTTCTG AAATCCAAGTATTGGAGCACTTGAACAGCACTGACCCCAACAGTGTCTTCCGATGCGTCCAGATGCTAGA GTGGTTTGATCATCATGGTCATGTTTGTATTGTGTTTGAGCTGCTGGGACTTAGTACCTATGATTTTATT AAAGAAAATAGTTTTCTGCCATTTCAAATTGATCACATCAGGCAAATGGCTTATCAGATCTGCCAGTCTA TAAATTTTTTACATCATAATAAATTAACACACACGGACCTAAAACCTGAAAATATTTTATTTGTGAAGTC TGACTATGTAGTCAAATACAATTCTAAAATGAAACGAGATGAGCGCACATTGAAAAACACAGATATCAAA GTTGTTGATTTTGGAAGTGCAACATATGACGACGAACATCATAGTACTTTGGTGTCCACAAGGCACTACA GGGCTCCAGAGGTCATTTTGGCTCTAGGTTGGTCTCAGCCTTGTGATGTTTGGAGCATAGGCTGCATTCT TATTGAGTACTACCTTGGGTTCACAGTCTTTCAGACCCACGATAGTAAAGAGCACCTGGCAATGATGGAG CGGATCTTAGGACCCATCCCAGCACATATGATCCAGAAGACAAGGAAACGCAAGTATTTCCACCATAACC AGCTAGATTGGGACGAGCATAGTTCAGCTGGGAGATATGTTAGGAGACGCTGCAAGCCGTTAAAGGAATT TATGCTGTGTCATGACGAAGAGCATGAGAAGCTGTTTGACCTGGTTCGAAGAATGTTGGAGTATGACCCA GCGAGAAGGATCACCTTGGATGAAGCATTGCAGCACCCTTTCTTTGACTTATTAAAAAGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204207 representing BC002220
Red=Cloning site Green=Tags(s) MDGLHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIVFELLGLSTYDFI KENSFLPFQIDHIRQMAYQICQSINFLHHNKLTHTDLKPENILFVKSDYVVKYNSKMKRDERTLKNTDIK VVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMME RILGPIPAHMIQKTRKRKYFHHNQLDWDEHSSAGRYVRRRCKPLKEFMLCHDEEHEKLFDLVRRMLEYDP ARRITLDEALQHPFFDLLKRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC002220 |
| ORF Size | 905 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002220, AAH02220 |
| RefSeq Size | 1445 bp |
| RefSeq ORF | 905 bp |
| Locus ID | 12750 |
| Gene Summary | Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204207 | Clk4 (Myc-DDK-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) |
CNY 2,400.00 |
|
| MR204207L3 | Lenti ORF clone of Clk4 (Myc-DDK-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) |
CNY 4,750.00 |
|
| MR204207L4 | Lenti ORF clone of Clk4 (mGFP-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) |
CNY 4,750.00 |
