Ppp6c (NM_024209) Mouse Tagged ORF Clone

CAT#: MG204292

  • TrueORF®

Ppp6c (tGFP-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c)

ORF Plasmid: DDK tGFP



  "NM_024209" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2310003C10Rik; Pp6C
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204292 representing NM_024209
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCTGGATCTGGACAAGTATGTGGAGATAGCGCGGCAGTGCAAGTACCTTCCCGAGAACGACC
TGAAGAGGCTATGTGACTATGTTTGTGATCTGCTCTTGGAAGAGTCGAATGTTCAGCCAGTGTCAACGCC
AGTAACAGTGTGTGGTGACATACACGGACAGTTTTATGATCTTTGTGAACTGTTCAGAACTGGAGGTCAG
GTTCCTGACACAAACTACATATTTATGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCA
CTTATCTTCTTGCACTAAAGGCTAAGTGGCCTGACCGTATTACACTTTTAAGAGGAAATCATGAGAGTAG
ACAGATAACACAGGTGTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
TACTGTACCAAAGTTTTTGATATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTGTGTGTTCATG
GCGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACTATTGAACGAAATCAGGAAATTCCTCA
CAAAGGAGCATTTTGTGACTTGGTGTGGTCAGATCCTGAAGATGTGGACACTTGGGCAATCAGTCCCAGA
GGAGCAGGTTGGCTATTTGGAGCAAAAGTCACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCT
GCAGAGCACATCAGCTTGTGCACGAAGGCTATAAGTTTATGTTTGACGAGAAGCTGGTCACAGTGTGGTC
TGCTCCTAACTACTGCTATCGCTGTGGAAACATTGCTTCCATCATGGTCTTCAAAGATGTCAATACGAGA
GAACCAAAGTTATTCCGAGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACCACGACGCCGTATT
TCCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204292 representing NM_024209
Red=Cloning site Green=Tags(s)

MAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQ
VPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWR
YCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPR
GAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTR
EPKLFRAVPDSERVIPPRTTTPYFL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024209
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024209.3
RefSeq Size 1608 bp
RefSeq ORF 918 bp
Locus ID 67857
UniProt ID Q9CQR6
Gene Summary Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. During mitosis, regulates spindle positioning. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. Participates also in the innate immune defense against viruses by desphosphorylating RIG-I/DDX58, an essential step that triggers RIG-I/DDX58-mediated signaling activation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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