Ppp6c (NM_024209) Mouse Tagged ORF Clone
CAT#: MG204292
- TrueORF®
Ppp6c (tGFP-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c)
ORF Plasmid: DDK
"NM_024209" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2310003C10Rik; Pp6C |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204292 representing NM_024209
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCTGGATCTGGACAAGTATGTGGAGATAGCGCGGCAGTGCAAGTACCTTCCCGAGAACGACC TGAAGAGGCTATGTGACTATGTTTGTGATCTGCTCTTGGAAGAGTCGAATGTTCAGCCAGTGTCAACGCC AGTAACAGTGTGTGGTGACATACACGGACAGTTTTATGATCTTTGTGAACTGTTCAGAACTGGAGGTCAG GTTCCTGACACAAACTACATATTTATGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCA CTTATCTTCTTGCACTAAAGGCTAAGTGGCCTGACCGTATTACACTTTTAAGAGGAAATCATGAGAGTAG ACAGATAACACAGGTGTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA TACTGTACCAAAGTTTTTGATATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTGTGTGTTCATG GCGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACTATTGAACGAAATCAGGAAATTCCTCA CAAAGGAGCATTTTGTGACTTGGTGTGGTCAGATCCTGAAGATGTGGACACTTGGGCAATCAGTCCCAGA GGAGCAGGTTGGCTATTTGGAGCAAAAGTCACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCT GCAGAGCACATCAGCTTGTGCACGAAGGCTATAAGTTTATGTTTGACGAGAAGCTGGTCACAGTGTGGTC TGCTCCTAACTACTGCTATCGCTGTGGAAACATTGCTTCCATCATGGTCTTCAAAGATGTCAATACGAGA GAACCAAAGTTATTCCGAGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACCACGACGCCGTATT TCCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204292 representing NM_024209
Red=Cloning site Green=Tags(s) MAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQ VPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWR YCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPR GAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTR EPKLFRAVPDSERVIPPRTTTPYFL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_024209 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024209.3 |
RefSeq Size | 1608 bp |
RefSeq ORF | 918 bp |
Locus ID | 67857 |
UniProt ID | Q9CQR6 |
Gene Summary | Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. During mitosis, regulates spindle positioning. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. Participates also in the innate immune defense against viruses by desphosphorylating RIG-I/DDX58, an essential step that triggers RIG-I/DDX58-mediated signaling activation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207641 | Ppp6c (untagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c), (10ug) |
CNY 3,990.00 |
|
MR204292 | Ppp6c (Myc-DDK-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c) |
CNY 2,400.00 |
|
MR204292L3 | Lenti ORF clone of Ppp6c (Myc-DDK-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c) |
CNY 4,750.00 |
|
MR204292L4 | Lenti ORF clone of Ppp6c (mGFP-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c) |
CNY 4,800.00 |