Cdk5r1 (BC050768) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	p35, Cdk5r, p25
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204342 representing BC050768 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACGGTGCTGTCCCTATCCCCCAGCTATCGGAAGGCCACACTGTTTGAGGATGGCGCGGCCACCG TGGGCCACTACACGGCCGTGCAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGGCACTCCATCAT CTCGGTGCTGCCTTGGAAGAGGATCGTGGCGGTGTCAGCGAAGAAGAAGAACTCCAAGAAGGCGCAGCCC AACAGCAGCTACCAGAGCAACATCGCGCATCTCAACAATGAGAACCTGAAGAAGTCGCTGTCCTGTGCCA ACCTGTCCACATTCGCCCAGCCCCCGCCGGCTCAGCCGCCCGCACCCCCAGCCAGCCAGCTTTCTGGCTC CCAGACTGGGGTCTCCTCTTCTGTCAAGAAGGCCCCGCACCCTGCCATCACCTCTGCAGGGACACCCAAA CGGGTCATCGTCCAGGCGTCCACTAGTGAGCTGCTGCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCT ACCGCCTGAAGCACTTGTCCCCAACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCCCTGCTTCT GCAGGGCTGGCAGGACCAGGGTTTCATCACACCGGCCAATGTGGTCTTCCTCTACATGCTCTGCAGGGAT GTCATCTCCTCTGAGGTGGGCTCCGACCATGAGCTCCAGGCTGTCCTGCTGACCTGTCTGTACCTCTCCT ATTCCTACATGGGCAATGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTAGAGAGCTGTAAGGAAGCCTT TTGGGACCGTTGCCTCTCAGTTATCAACCTCATGAGCTCCAAGATGCTGCAGATCAATGCCGACCCACAC TATTTCACACAAGTGTTCTCTGACTTGAAGAATGAGAGCGGTCAGGAGGACAAGAAGCGACTCCTCCTGG GGCTGGATCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204342 representing BC050768 Red=Cloning site Green=Tags(s) MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKAQP NSSYQSNIAHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGVSSSVKKAPHPAITSAGTPK RVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRD VISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPH YFTQVFSDLKNESGQEDKKRLLLGLDR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050768
ORF Size	921 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050768, AAH50768
RefSeq Size	4166 bp
RefSeq ORF	923 bp
Locus ID	12569
Gene Summary	p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.[UniProtKB/Swiss-Prot Function]

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