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Rnf126 (NM_144528) Mouse Tagged ORF Clone
CAT＃: MG204456
- TrueORF®
Rnf126 (tGFP-tagged) - Mouse ring finger protein 126 (Rnf126)
ORF Plasmid: DDK
"NM_144528" in other vectors (3)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610010O19Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204456 representing NM_144528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCGTCGCCGCAGCCCGGACGGTACTTCTGCCACTGCTGCTCGGTAGAGATCGTGCCGCGCC TGCCGGATTACATCTGCCCAAGGTGCGAGTCTGGCTTCATTGAGGAGCTTCCAGAAGAGACCAGGAACAC AGAGAATGGCTCAGCCCCCTCCACAGCCCCCACCGACCAGAACCGGCAGCCATTTGAGAATGTGGACCAG CACCTGTTCACGCTGCCACAGGGATACAGCCAGTTTGCCTTTGGCATCTTCGACGATAGCTTTGAGATTC CCACGTTCCCTCCTGGAGCCCAGGCCGATGATGGCAGGGACCCTGAGAGCCGACGGGAGAGAGAGCACCA GTCTCGGCATCGGTACGGGGCCCGGCAGCCCCGTGCCCGCCTCACTGCCCGCCGGGCCACTGGCCGGCAT GAAGGTGTCCCTACGCTGGAAGGGATCATCCAGCAGCTCGTGAATGGCATCATCTCTCCGGCTGCTGTGC CCAGCCTGGGCCTTGGTCCCTGGGGCGTCCTGCACTCGAACCCAATGGACTACGCCTGGGGGGCCAACGG CCTGGACACCATCATCACGCAGCTCCTCAATCAGTTTGAGAACACCGGCCCCCCACCTGCAGACAAGGAG AAGATTCAGGCTCTCCCCACGGTCCCAGTCACAGAGGAACACGTGGGCTCAGGGCTTGAGTGCCCAGTGT GTAAAGAAGACTATGCACTGGGTGAGAGTGTGCGGCAGTTGCCCTGCAACCACCTGTTCCACGACAGCTG CATCGTGCCCTGGCTGGAGCAGCATGACAGCTGCCCGGTCTGCCGTAAGAGCCTCACTGGACAGAACACA GCCACCAATCCCCCAGGCTTGACCGGTGTAGGTTTCTCCTCGTCCTCGTCCTCATCCTCCTCCAGCTCGC CCAGCAATGAGAACGCCACAAGCAACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204456 representing NM_144528
Red=Cloning site Green=Tags(s) MAEASPQPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRNTENGSAPSTAPTDQNRQPFENVDQ HLFTLPQGYSQFAFGIFDDSFEIPTFPPGAQADDGRDPESRREREHQSRHRYGARQPRARLTARRATGRH EGVPTLEGIIQQLVNGIISPAAVPSLGLGPWGVLHSNPMDYAWGANGLDTIITQLLNQFENTGPPPADKE KIQALPTVPVTEEHVGSGLECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCRKSLTGQNT ATNPPGLTGVGFSSSSSSSSSSSPSNENATSNS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144528 |
| ORF Size | 939 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144528.3 |
| RefSeq Size | 1619 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 70294 |
| UniProt ID | Q91YL2 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination oF target proteins (By similarity). Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates (PubMed:23418353). Part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome (By similarity). Probably acts by providing the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the hydrophobic mislocalized proteins and their targeting to the proteasome (By similarity). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (By similarity). May play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4, by mediating their ubiquitination (PubMed:23418353). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (By similarity). May monoubiquitinate AICDA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204456 | Rnf126 (Myc-DDK-tagged) - Mouse ring finger protein 126 (Rnf126), transcript variant 1 |
CNY 2,400.00 |
|
| MR204456L3 | Lenti ORF clone of Rnf126 (Myc-DDK-tagged) - Mouse ring finger protein 126 (Rnf126), transcript variant 1 |
CNY 4,750.00 |
|
| MR204456L4 | Lenti ORF clone of Rnf126 (mGFP-tagged) - Mouse ring finger protein 126 (Rnf126), transcript variant 1 |
CNY 4,750.00 |
