Slc9a3r2 (BC070458) Mouse Tagged ORF Clone

CAT#: MG204526

  • TrueORF®

Slc9a3r2 (tGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578)

ORF Plasmid: DDK tGFP



  "BC070458" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Slc9a3r2 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms E3karp, Sip-1, Tka-1, Octs2, NHERF-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204526 representing BC070458
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGCTGGAATCGCTGCGGCCCCGCTTGTGTCGCCTGGTGCGCGGAGAGCAAGGGTACGGCTTTC
ACCTGCATGGCGAGAAAGGCCGCCGTGGCCAGTTCATCCGGCGCGTGGAGCCAGGCTCCCCAGCCGAGGC
GGCCGCGCTGCGCGCCGGGGACCGCCTGGTTGAGGTCAACGGCGTCAACGTAGAGGGCGAGACGCATCAC
CAGGTGGTACAGAGGATCAAGGCTGTGGAGGGGCAGACCCAGCTGCTTGTGGTGGACAAGGAGACCGATG
AGGAGCTCTGCCGGCGGCAACTGACCTGCACTGAGGAGATGGCCCATCGAGGGCTTCCCCCAGCCCACAA
CCCCTGGGAGCCAAAGCCGGACTGGGCATGCTCGGGCAGCCTTGGCTCGGACACTGGCCAGAAGGATGTC
AATGGGCCCCCAAGAGAGCTGCGTCCCCGGCTCTGCCACTTGCGAAGGGGACCCCAGGGCTACGGGTTCA
ACCTGCATAGTGACAAGTCCCGGCCTGGCCAGTACATCCGCTCTGTGGACCCTGGCTCTCCTGCTTCTCA
CTCTGGCCTCCGTGCCCAGGATCGGCTCATTGAGGTGAATGGGCAGAACGTGGAGGGGCTGCGACATGCC
GAGGTTGTCGCCAGAATCAAGGCACAGGAGGACGAGGCCCGGCTGCTGGTAGTCGATCCTGAGACTGATG
AACACTTCAAGCGGTTGAGGGTCATACCCACTGAGGAACATGTAGAAGGTCCACTGCCATCACCAGTCAC
GAACGGGACCAGTCCTGCCCAGGATGGCAGTACCTGGAAGCGAGACCCCTTCCAGGAAAGTGGCCTCCAC
CTGAGTCCCACAGCAGCTGAGGCCAAGGAGAAGGCTCGAGCTACTCGGGTCAACAAGCGGGCACCACAGA
TGGACTGGAACCGGAAGCGAGAGATCTTCAGCAACTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204526 representing BC070458
Red=Cloning site Green=Tags(s)

MAALESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHH
QVVQRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGQKDV
NGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVDPGSPASHSGLRAQDRLIEVNGQNVEGLRHA
EVVARIKAQEDEARLLVVDPETDEHFKRLRVIPTEEHVEGPLPSPVTNGTSPAQDGSTWKRDPFQESGLH
LSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC070458
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC070458, AAH70458
RefSeq Size 2052 bp
RefSeq ORF 950 bp
Locus ID 65962
Gene Summary Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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