Acy3 (BC010795) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	HCBP1, Acy-3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204580 representing BC010795 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCCTACCTGGGTCCCGGGAGCCCCTGCTCCGTGTGGCTGTGACTGGAGGCACCCACGGGAATG AGATGTGTGGTGTCTACCTGGCCCGGTACTGGCTACAGAACCCAGGGGAGCTGCAGAGACCCAGCTTCTC AGCCATGCCGGTTCTGGCCAACCCAGCAGCCACAGCTGCCTGTTGCCGTTACCTGGACCGTGATCTCAAC CGCTCCTGCACCCTCACCTTCCTTGGTTCCACCGCTACCCCTGATGACCCCTATGAAGTGAAAAGAGCCC GAGAGTTGAACCAGCTGCTGGGTCCCAAGGGCACAGGCCAGGCTTTTGACTTTACCCTAGACCTGCACAA CACCACAGCAAACACTGGAGTCTGCCTCATCTCTGAATCCAACATCTCCTTCAACTTGCACCTGTGCCAC TACCTACAGCGGCAGAACCCGGGGATGCCCTGCCGCCTCTTTCTGTATGAGCCAGCTGGGACGGAGACCT TCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAGCCTCAGGGCGTGCTGCG GGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAA GGCATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTG CGGATGGTGACCTGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGG TGAACCCATCTTCAAGCTTTTCAGCGGAGAAGACGTACTGTATGAGGGGGACTCCATTGTGTACCCTGTG TTCATTAATGAGGCTGCCTATTATGAGAAGCACGTGGCATTCCTGAAGTCTGAGAAGATCAGGGTCACAG TGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204580 representing BC010795 Red=Cloning site Green=Tags(s) MSSLPGSREPLLRVAVTGGTHGNEMCGVYLARYWLQNPGELQRPSFSAMPVLANPAATAACCRYLDRDLN RSCTLTFLGSTATPDDPYEVKRARELNQLLGPKGTGQAFDFTLDLHNTTANTGVCLISESNISFNLHLCH YLQRQNPGMPCRLFLYEPAGTETFSVESISKNGICLEMGPQPQGVLRADLFSRMRALVASILDFIELFNQ GMDLPAFEMDIYRNLGSVDFPRTADGDLAGTVHPQLQDHDFEPLRPGEPIFKLFSGEDVLYEGDSIVYPV FINEAAYYEKHVAFLKSEKIRVTVPALLRLTPRSTQTP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC010795
ORF Size	956 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010795, AAH10795
RefSeq Size	1531 bp
RefSeq ORF	956 bp
Locus ID	71670
Gene Summary	This gene encodes a member of the aminoacylase family of enzymes. This enzyme specifically deacetylates N-acetyl aromatic amino acids and mercapturic acids. Action of this enzyme on metabolites of the environmental contaminant trichloroethylene leads to the generation of toxic products that may lead to kidney failure. This protein has been found to bind to the hepatitis C virus core protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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