Icosl (NM_015790) Mouse Tagged ORF Clone
CAT#: MG204667
- TrueORF®
Icosl (tGFP-tagged) - Mouse icos ligand (Icosl)
ORF Plasmid: DDK
"NM_015790" in other vectors (4)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AU044799; B7-H2; B7h; B7RP-1; BG071784; GI50; GL50; GL50-B; ICOS-L; Icoslg; LICOS; Ly115l |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204667 representing NM_015790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTAAAGTGTCCCTGTTTTGTGTCCTTGGGAACCAGGCAGCCTGTTTGGAAGAAGCTCCATGTTT CTAGCGGGTTCTTTTCTGGTCTTGGTCTGTTCTTGCTGCTGTTGAGCAGCCTCTGTGCTGCCTCTGCAGA GACTGAAGTCGGTGCAATGGTGGGCAGCAATGTGGTGCTCAGCTGCATTGACCCCCACAGACGCCATTTC AACTTGAGTGGTCTGTATGTCTATTGGCAAATCGAAAACCCAGAAGTTTCGGTGACTTACTACCTGCCTT ACAAGTCTCCAGGGATCAATGTGGACAGTTCCTACAAGAACAGGGGCCATCTGTCCCTGGACTCCATGAA GCAGGGTAACTTCTCTCTGTACCTGAAGAATGTCACCCCTCAGGATACCCAGGAGTTCACATGCCGGGTA TTTATGAATACAGCCACAGAGTTAGTCAAGATCTTGGAAGAGGTGGTCAGGCTGCGTGTGGCAGCAAACT TCAGTACACCTGTCATCAGCACCTCTGATAGCTCCAACCCGGGCCAGGAACGTACCTACACCTGCATGTC CAAGAATGGCTACCCAGAGCCCAACCTGTATTGGATCAACACAACGGACAATAGCCTAATAGACACGGCT CTGCAGAATAACACTGTCTACTTGAACAAGTTGGGCCTGTATGATGTAATCAGCACATTAAGGCTCCCTT GGACATCTCGTGGGGATGTTCTGTGCTGCGTAGAGAATGTGGCTCTCCACCAGAACATCACTAGCATTAG CCAGGCAGAAAGTTTCACTGGAAATAACACAAAGAACCCACAGGAAACCCACAATAATGAGTTAAAAGTC CTTGTCCCCGTCCTTGCTGTACTGGCGGCAGCGGCATTCGTTTCCTTCATCATATACAGACGCACGCGTC CCCACCGAAGCTATACAGGACCCAAGACTGTACAGCTTGAACTTACAGACCACGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204667 representing NM_015790
Red=Cloning site Green=Tags(s) MQLKCPCFVSLGTRQPVWKKLHVSSGFFSGLGLFLLLLSSLCAASAETEVGAMVGSNVVLSCIDPHRRHF NLSGLYVYWQIENPEVSVTYYLPYKSPGINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRV FMNTATELVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLYWINTTDNSLIDTA LQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENVALHQNITSISQAESFTGNNTKNPQETHNNELKV LVPVLAVLAAAAFVSFIIYRRTRPHRSYTGPKTVQLELTDHA SGPTRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_015790 |
ORF Size | 966 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_015790.3, NP_056605.1 |
RefSeq Size | 2748 bp |
RefSeq ORF | 969 bp |
Locus ID | 50723 |
UniProt ID | Q9JHJ8 |
Gene Summary | Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202216 | Icosl (untagged) - Mouse icos ligand (Icosl), (10ug) |
CNY 2,400.00 |
|
MR204667 | Icosl (Myc-DDK-tagged) - Mouse icos ligand (Icosl) |
CNY 2,400.00 |
|
MR204667L3 | Lenti ORF clone of Icosl (Myc-DDK-tagged) - Mouse icos ligand (Icosl) |
CNY 4,750.00 |
|
MR204667L4 | Lenti ORF clone of Icosl (mGFP-tagged) - Mouse icos ligand (Icosl) |
CNY 4,800.00 |