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Tsfm (NM_025537) Mouse Tagged ORF Clone
CAT＃: MG204717
- TrueORF®
Tsfm (tGFP-tagged) - Mouse Ts translation elongation factor, mitochondrial (Tsfm)
ORF Plasmid: DDK
"NM_025537" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310050B20Rik; 9430024O13Rik; EF-TS; EF-Tsmt |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204717 representing NM_025537
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTACTGCGGTCGCTGCGCTTCTTCCCGGTCGCGTGCACCGGCCGCTCGGCGAGGGCTGTCCTGC TCCAGCCGTCCCAGCCATGGCTCACGTTTCATGCCGGCCCCTCGCTGTCCTCTGCTGCCTCCAGCAAGGA GCTCCTTATGAAACTGCGGCGGAAAACCGGCTACTCCTTTGTCAACTGCAAGAAGGCTTTGGAGACTTGT GGCGGTGACCTCAAGCAGGCAGAGGACTGGCTGCACAAACAGGCCCAGAAGGAAGGATGGAGCAAAGCCG CCAAGCTCCACGGGAGGAAGACTAAAGAAGGCTTGATTGGACTTCTGCAGGAGGGAAACACAGCTGTTTT AGTAGAGGTAAACTGTGAGACAGATTTTGTCTCCAGAAATTTAAAATTTCAACAGTTGGTCCAGCAAGTA GCCCTGGGAACCATGGCACACTGTCAGAACCTGACGGATCGGCTCTCCACGTACAGTAAGGGCTTTCTCA ACTCCTCTGAGCTTTCTGAACTTGCAGCTGGGCCCGACAGGGAAGGCTCTCTCAAGGATCAGTTGGCCCT AGCGATTGGTAAACTGGGAGAGAACATGATTCTTAAGCGAGCTGCCTGGGTGAAGGTGCCCTCTGGGTTC TATGTCGGCTCTTATGTGCACGGAGTGACGCAGAGCCCCTCCCTCCAGAACCTGGTGCTGGGGAAGTACG GGGCCCTGGTCATCTGTGAGACTCCCGAGCAGATCGCAAACCTGGAGGAGGTTGGCCGCCGCTTGGGGCA GCACGTGGTGGGCATGGCCCCTCTCTCTGTGGGCTCCCTGGACGATGAGCCTGGGGGGGAGACGGAGACC AGGATGCTGCCCCAGCCGTACCTCCTGGATCCTTCCATTACACTGGGGCAGTACGTGCAGCCCCAGGGCG TGACTGTAGTGGACTTCGTGCGCTTCGAATGTGGAGAAGACGAACAGGTGGCCGAGGCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204717 representing NM_025537
Red=Cloning site Green=Tags(s) MSLLRSLRFFPVACTGRSARAVLLQPSQPWLTFHAGPSLSSAASSKELLMKLRRKTGYSFVNCKKALETC GGDLKQAEDWLHKQAQKEGWSKAAKLHGRKTKEGLIGLLQEGNTAVLVEVNCETDFVSRNLKFQQLVQQV ALGTMAHCQNLTDRLSTYSKGFLNSSELSELAAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGF YVGSYVHGVTQSPSLQNLVLGKYGALVICETPEQIANLEEVGRRLGQHVVGMAPLSVGSLDDEPGGETET RMLPQPYLLDPSITLGQYVQPQGVTVVDFVRFECGEDEQVAEAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025537 |
| ORF Size | 972 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025537.2 |
| RefSeq Size | 1180 bp |
| RefSeq ORF | 975 bp |
| Locus ID | 66399 |
| UniProt ID | Q9CZR8 |
| Gene Summary | Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203293 | Tsfm (untagged) - Mouse Ts translation elongation factor, mitochondrial (Tsfm), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,400.00 |
|
| MR204717 | Tsfm (Myc-DDK-tagged) - Mouse Ts translation elongation factor, mitochondrial (Tsfm), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
| MR204717L3 | Lenti ORF clone of Tsfm (Myc-DDK-tagged) - Mouse Ts translation elongation factor, mitochondrial (Tsfm), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR204717L4 | Lenti ORF clone of Tsfm (mGFP-tagged) - Mouse Ts translation elongation factor, mitochondrial (Tsfm), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
