Cdk10 (BC017131) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	BC017131
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204870 representing BC017131 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGGAAGATGCCGAAGCGTTAAGGAGTTTGAGAAGCTGAACCGGATTGGCGAGGGCACCTATGGCATCG TGTATCGGGCCAGGGATACCCAGACAGATGAAATTGTCGCCCTGAAGAAGGTGCGGATGGACAAAGAGAA GGATGGCATCCCCATCAGCAGCCTGCGTGAGATCACACTGCTCTTGCGTCTCCGCCATCCAAACATTGTG GAGCTGAAGGAGGTGGTTGTGGGCAACCACCTGGAGAGCATCTTCCTGGTCATGGGTTACTGCGAACAAG ATCTGGCCAGCCTATTGGAAAATATGCCAACACCCTTCTCGGAGGCCCAGGTTAAATGCATCATGCTACA GGTGCTTCGTGGCCTTCAGTACCTGCACAGGAACTTCATCATCCACAGGGACCTGAAGGTGTCCAACTTG CTCATGACAGACAAGGGCTGTGTAAAGACAGCTGATTTTGGCCTGGCTCGGGCCTATGGTGTTCCAGTAA AGCCAATGACTCCCAAGGTTGTTACCCTCTGGTACCGAGCCCCAGAGCTGCTGCTTGGAACTACCACCCA GACTACCAGCATTGACATGTGGGCTGTCGGCTGCATCCTGGCAGAGCTGCTGGCCCATAAGCCCCTCCTC CCTGGCACTTCCGAGATCCACCAGATCGACTTGATTGTACAGCTGTTGGGGACACCGAGTGAGAATATCT GGCCGGGTTTCTCCAAGCTGCCGCTGGCCGGCCAGTACAGCTTGAGGAAACAGCCCTATAACAACCTCAA GCACAAGTTCCCGTGGCTCTCAGAGGCCGGACTCCGTCTGCTCAACTTCCTCTTCATGTATGACCCTAAG AAAAGGGCAACCTCGGGAGACTGCCTGGAGAGCTCCTACTTCAAGGAGAAGCCCCTGCCCTGTGAACCGG AGCTCATGCCTACCTTCCCCCACCACCGCAATAAGCGTGCTGCCCCAGCTGCCGCTGAAGGGCAGAGCAA ACGATGCCGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204870 representing BC017131 Red=Cloning site Green=Tags(s) LGRCRSVKEFEKLNRIGEGTYGIVYRARDTQTDEIVALKKVRMDKEKDGIPISSLREITLLLRLRHPNIV ELKEVVVGNHLESIFLVMGYCEQDLASLLENMPTPFSEAQVKCIMLQVLRGLQYLHRNFIIHRDLKVSNL LMTDKGCVKTADFGLARAYGVPVKPMTPKVVTLWYRAPELLLGTTTQTTSIDMWAVGCILAELLAHKPLL PGTSEIHQIDLIVQLLGTPSENIWPGFSKLPLAGQYSLRKQPYNNLKHKFPWLSEAGLRLLNFLFMYDPK KRATSGDCLESSYFKEKPLPCEPELMPTFPHHRNKRAAPAAAEGQSKRCRP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC017131
ORF Size	993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017131, AAH17131
RefSeq Size	1599 bp
RefSeq ORF	995 bp
Locus ID	234854
Gene Summary	The protein encoded by this gene belongs to the CDK (cyclin-dependent kinase) subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. The human ortholog has been shown to play a role in cellular proliferation. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 1. [provided by RefSeq, Jan 2010]

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