Osgep (BC002296) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	GCPL-1, PRSMG1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204973 representing BC002296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCGGTGCTGGGGTTCGAAGGCAGCGCCAACAAGATCGGCGTGGGCGTGGTCCGCGACGGCACGG TGCTGGCGAACCCGCGGCGCACTTACGTCACGGCCCCGGGCACGGGATTCCTTCCAGGTGACACGGCCAG GCACCATCGAGCTGTTATCCTAGACCTACTGCAGGAGGCGCTAGCAGAGGCAGGATTGACCTCCAAGGAC ATTGATTGCATTGCTTTCACCAAAGGTCCTGGCATGGGAGCCCCATTGGCTTCTGTAGCTGTTGTTGCCC GTACAGTGGCCCAGTTGTGGAATAAGCCTTTGCTTGGCGTGAACCACTGCATAGGCCACATTGAAATGGG CCGTCTCATCACTGGAGCCGTTAACCCAACTGTCCTGTATGTGAGCGGAGGAAATACCCAGGTGATTTCC TACTCAGAACATCGTTATCGCATCTTTGGAGAAACTATTGATATCGCCGTGGGAAACTGCCTGGATCGTT TTGCTCGGGTGCTGAAGATTTCCAATGACCCCAGTCCAGGCTACAACATTGAGCAGATGGCAAAGCGAGG CAAGAAGCTAGTCGAGCTGCCATATACTGTAAAGGGGATGGATGTCTCGTTTTCAGGGATTCTGTCTTTC ATTGAGGATGCAGCGCAGCGAATGCTGGCCACTGGAGAGTGTACTCCTGAAGACCTGTGTTTCTCCTTAC AGGAAACCGTGTTTGCAATGCTAGTGGAAATCACGGAGCGAGCCATGGCACACTGTGGCTCCAAGGAAGC CCTCATCGTCGGAGGAGTTGGATGTAACCTGAGGCTGCAGGAGATGATGGGGACAATGTGCCAGGAGCGG GGAGCCCAGCTCTTTGCAACAGATGAGAGATTCTGCGTTGACAATGGAGCCATGATAGCACAAGCTGGTT GGGAGATGTTTCAGGCTGGGCACAGGACTCCTCTCAAAGATTCTGCAATTACTCAGAGGTATAGGACAGA TGAAGTGGAAGTGACATGGAGGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204973 representing BC002296 Red=Cloning site Green=Tags(s) MPAVLGFEGSANKIGVGVVRDGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALAEAGLTSKD IDCIAFTKGPGMGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVIS YSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSF IEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVGGVGCNLRLQEMMGTMCQER GAQLFATDERFCVDNGAMIAQAGWEMFQAGHRTPLKDSAITQRYRTDEVEVTWRD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002296
ORF Size	1007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002296, AAH02296
RefSeq Size	1248 bp
RefSeq ORF	1007 bp
Locus ID	66246
Gene Summary	Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.[UniProtKB/Swiss-Prot Function]

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