Osgep (BC002296) Mouse Tagged ORF Clone

CAT#: MG204973

  • TrueORF®

Osgep (tGFP-tagged) - Mouse O-sialoglycoprotein endopeptidase (cDNA clone MGC:8193 IMAGE:3590664)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms GCPL-1, PRSMG1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204973 representing BC002296
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGCGGTGCTGGGGTTCGAAGGCAGCGCCAACAAGATCGGCGTGGGCGTGGTCCGCGACGGCACGG
TGCTGGCGAACCCGCGGCGCACTTACGTCACGGCCCCGGGCACGGGATTCCTTCCAGGTGACACGGCCAG
GCACCATCGAGCTGTTATCCTAGACCTACTGCAGGAGGCGCTAGCAGAGGCAGGATTGACCTCCAAGGAC
ATTGATTGCATTGCTTTCACCAAAGGTCCTGGCATGGGAGCCCCATTGGCTTCTGTAGCTGTTGTTGCCC
GTACAGTGGCCCAGTTGTGGAATAAGCCTTTGCTTGGCGTGAACCACTGCATAGGCCACATTGAAATGGG
CCGTCTCATCACTGGAGCCGTTAACCCAACTGTCCTGTATGTGAGCGGAGGAAATACCCAGGTGATTTCC
TACTCAGAACATCGTTATCGCATCTTTGGAGAAACTATTGATATCGCCGTGGGAAACTGCCTGGATCGTT
TTGCTCGGGTGCTGAAGATTTCCAATGACCCCAGTCCAGGCTACAACATTGAGCAGATGGCAAAGCGAGG
CAAGAAGCTAGTCGAGCTGCCATATACTGTAAAGGGGATGGATGTCTCGTTTTCAGGGATTCTGTCTTTC
ATTGAGGATGCAGCGCAGCGAATGCTGGCCACTGGAGAGTGTACTCCTGAAGACCTGTGTTTCTCCTTAC
AGGAAACCGTGTTTGCAATGCTAGTGGAAATCACGGAGCGAGCCATGGCACACTGTGGCTCCAAGGAAGC
CCTCATCGTCGGAGGAGTTGGATGTAACCTGAGGCTGCAGGAGATGATGGGGACAATGTGCCAGGAGCGG
GGAGCCCAGCTCTTTGCAACAGATGAGAGATTCTGCGTTGACAATGGAGCCATGATAGCACAAGCTGGTT
GGGAGATGTTTCAGGCTGGGCACAGGACTCCTCTCAAAGATTCTGCAATTACTCAGAGGTATAGGACAGA
TGAAGTGGAAGTGACATGGAGGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204973 representing BC002296
Red=Cloning site Green=Tags(s)

MPAVLGFEGSANKIGVGVVRDGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALAEAGLTSKD
IDCIAFTKGPGMGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVIS
YSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSF
IEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVGGVGCNLRLQEMMGTMCQER
GAQLFATDERFCVDNGAMIAQAGWEMFQAGHRTPLKDSAITQRYRTDEVEVTWRD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC002296
ORF Size 1007 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC002296, AAH02296
RefSeq Size 1248 bp
RefSeq ORF 1007 bp
Locus ID 66246
Gene Summary Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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