Ubiad1 (BC071203) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1200002M06Rik; AI426463; AW320947; Tere1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204986 representing BC071203 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGTACAGGCCCCGGGGGAGAAGATTAATATCCTGGCAGGAGAGACAGCCAAGGTCGGGGACC CGCAGAAGAACGAATGGCCCGAGCAGGACAGGTTTCCTGAACGATCCTGGAGGCACAAGTGCGCCTCCTA CGTGTTGGCCCTGAGGCCCTGGAGCTTCAGTGCCTCACTCACCCCTGTGGCCCTGGGCAGTGCCTTGGCC TACAGGTCTCAGGGTGTCCTGGATCCCAGGCTGTTGTTGGGTTGTGCAGTGGCTGTCCTGGCTGTGCACG GGGCCGGCAATTTGGTCAACACATACTATGACTTTTCCAAGGGCATTGACCACAAAAAGAGTGATGACAG AACTTTGGTGGACAGAATTCTGGAGCCCCAGGATGTTGTTCGATTTGGAGTCTTCCTCTACACCTTGGGC TGCGTCTGTGCTGCTTGCCTCTACTACTTGTCCGCTCTGAAATTGGAACACTTGGCTCTCATCTACTTCG GAGGCCTGTCTGGCTCCTTTCTCTACACAGGAGGAATTGGATTCAAGTATGTGGCCCTGGGAGACCTCGT CATCCTCATCACTTTCGGCCCGCTGGCTGTGATGTTTGCCTACGCTGTCCAGGTGGGATCCCTGGCCATC TTCCCTCTAATCTACGCCATCCCTCTGGCCCTCAGCACGGAGGCCATTCTCCATTCCAACAACACCAGGG ACATGGAATCTGACCGAGAGGCTGGCATCGTCACGCTGGCCATCCTCATTGGGCCCACCTTCTCCTATGT CCTCTATAACACACTGCTCTTTGTGCCCTACCTAATCTTTACCATCCTGGCCACGCACTGCAGCATCAGC CTGGCACTGCCCCTGCTCACCATCCCCATGGCCTTCTCCCTTGAGAGGCAGTTCCGCAGCCAGGCCTTCA ACAAGCTGCCCCAGAGGACAGCCAAGCTCAACCTCCTGCTGGGGCTTTTCTATGTCTTTGGCATCATCCT GGCACCAGCAGGCAGCCTGCCCAGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204986 representing BC071203 Red=Cloning site Green=Tags(s) MAAVQAPGEKINILAGETAKVGDPQKNEWPEQDRFPERSWRHKCASYVLALRPWSFSASLTPVALGSALA YRSQGVLDPRLLLGCAVAVLAVHGAGNLVNTYYDFSKGIDHKKSDDRTLVDRILEPQDVVRFGVFLYTLG CVCAACLYYLSALKLEHLALIYFGGLSGSFLYTGGIGFKYVALGDLVILITFGPLAVMFAYAVQVGSLAI FPLIYAIPLALSTEAILHSNNTRDMESDREAGIVTLAILIGPTFSYVLYNTLLFVPYLIFTILATHCSIS LALPLLTIPMAFSLERQFRSQAFNKLPQRTAKLNLLLGLFYVFGIILAPAGSLPRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC071203
ORF Size	1010 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC071203, AAH71203
RefSeq Size	2976 bp
RefSeq ORF	1010 bp
Locus ID	71707
Gene Summary	Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays an important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function]

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