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H2 (BC080812) Mouse Tagged ORF Clone
CAT＃: MG205115
- TrueORF®
H2 (tGFP-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887)
ORF Plasmid: DDK
"BC080812" in other vectors (4)
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CNY 2,090.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Q1b, Q1d, Q1k |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205115 representing BC080812
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTGGGAAGGCTGCTCCTGCTGCTGGCAGCCGCCCTGACCCTGACCAAAACCGGAGCGGGCTCAC ACTCGCTGCGGTATTTCGAGACCTCGGTGTCCCAGCCCGGCTTCGGGAAGCCCCGGTTCATCTCTGTCGG CTACGTGGACGACACGCAGTTTGTGCGCTTCGACAGCGACGCGAAGAATCCGAGATATGAGCTGCGGGCG CCGTGGATGGAGCAGGAGGGGCCGGAGTATTGGGAGCGGAACACACGGAGAGTCAAGGGCAGTGAGAAGA GATTCCAAGAGAGCCTGAGCACCCTGCTCAGCTACTACAACCAGAGCAAGGGCGGCATTCACACCTTCCA GAAGTTGTCTGGCTGTGATCTGGGGTCAGATGGGCGCCTTCAAAGCGGGTACCTGCAGTTCGCCTATGAT GGCCTTGATTACATCGCCCTGAATGAAGACCTGGAAACCTGGACAGCAGCAGATGTGGCAGCTCAGGAAA CCCGACACAAGTGGGAGCAGGCTGGTGCTGCTGAGAAACACAGGACCTACCTGGAGGGCAAGTGCCTGAT GTGGCTCCACAGATACCTGGAGCTCAGGAAGGAGATGCTGCTGCGCACAGATCCCCCAAAGGCACATGTG ACTCATCACCCCAGATCTCAAGGTGATGTCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCTGACA TCACCCTGACCTGGCAGTTGAATGGGGAGGAGCTGACCCAGCACATGGAGCTTGTGGAGACCAGGCCTGC AGGGGATGGAACCTTCCAGAAGTGGGCATCTGTGGTGGTGCCTCTTGGGAAGGAGCAGAATTACACATGC CATGTGTACCATGAGGGGCTGCCTGAGCCCCTCACCCTGAGATGGGAGCCTCCTCCATACACTGTTTCCA ACATGGTAATCATAGCTGTTCTGGTTGTCCTTGGAGCTGTGATAGTCATTGGAGCTGTGGTCATCATTGG AGTTATGGTGTCTTTTGTGATGAAGAGGAGGAGAAACAAAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205115 representing BC080812
Red=Cloning site Green=Tags(s) MALGRLLLLLAAALTLTKTGAGSHSLRYFETSVSQPGFGKPRFISVGYVDDTQFVRFDSDAKNPRYELRA PWMEQEGPEYWERNTRRVKGSEKRFQESLSTLLSYYNQSKGGIHTFQKLSGCDLGSDGRLQSGYLQFAYD GLDYIALNEDLETWTAADVAAQETRHKWEQAGAAEKHRTYLEGKCLMWLHRYLELRKEMLLRTDPPKAHV THHPRSQGDVTLRCWALGFYPADITLTWQLNGEELTQHMELVETRPAGDGTFQKWASVVVPLGKEQNYTC HVYHEGLPEPLTLRWEPPPYTVSNMVIIAVLVVLGAVIVIGAVVIIGVMVSFVMKRRRNKA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC080812 |
| ORF Size | 1025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC080812, AAH80812 |
| RefSeq Size | 2001 bp |
| RefSeq ORF | 1025 bp |
| Locus ID | 15006 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218176 | H2 (untagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887), (10ug) |
CNY 6,940.00 |
|
| MR205115 | H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) |
CNY 3,656.00 |
|
| MR205115L3 | Lenti ORF clone of H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) |
CNY 3,800.00 |
|
| MR205115L4 | Lenti ORF clone of H2 (mGFP-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) |
CNY 3,800.00 |
