Bdh1 (BC096457) Mouse Tagged ORF Clone
CAT#: MG205162
- TrueORF®
Bdh1 (tGFP-tagged) - Mouse 3-hydroxybutyrate dehydrogenase, type 1 (cDNA clone MGC:106120 IMAGE:4975979)
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CNY 3,140.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2310032J20Rik; AI327223; Bdh |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205162 representing BC096457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCTGCCCGTCTCTCCAGACCCCTGTCACAGCTCCCAGGAAAAGCCCTAAGTGTCCGTGACAGAG AAAATGGGACAAGACACACGCTGTTGTTTTATCCAGCATCCTTCAGCCCTGACACCCGTCGGACCTACGC CAGTCAGGCAGATGCGGCTAGTGGCAAAGCTATCCTGATCACAGGCTGTGACTCTGGATTTGGGTTCTCA CTGGCCAAGCATCTGCACTCAAAAGGCTTCCTTGTATTTGCTGGCTGTTTGATGAAGGACAAAGGTGATG CTGGGGTCAAGGAACTGGACAGCTTGAAGAGTGACCGACTGAGAACCATCCAGCTCAATGTCTGCAACAG TGAAGAGGTGGAGAAGGCGGTGGAGACGATCCGCTCCGGCCTGAAAGATCCTGAGAAGGGAATGTGGGGC CTGGTTAACAACGCAGGCATCTCAACGTTTGGGGAGGTGGAGTTCACCAGCATGGAGACATATAAGGAGG TGGCTGAAGTGAACCTCTGGGGAACCGTGCGCACCACAAAATCCTTCCTTCCCCTTCTCCGAAGAGCCAA AGGTCGCGTCGTTAACATCAGCAGCATGCTGGGCCGCATGGCCAACCCCGCCCGCTCGCCATACTGCATC ACCAAGTTTGGGGTCGAGGCTTTCTCGGACTGCCTGCGCTATGAGATGCACCCTCTGGGTGTCAAGGTCA GTGTGGTGGAACCTGGCAACTTCATAGCGGCCACCAGTCTCTACAGCCCCGAGCGCATCCAGGCCATCGC CAAGAAGATGTGGGATGACCTGCCTGAGGTCGTCCGCAAGGACTATGGCAGGAAGTACTTCGATGAAAAG ATTGCCAAGATGGAAACCTACTGCAACAGCGGTTCCACAGATACTTCCTCTGTCATCAACGCTGTCACAC ACGCCTTGACCGCCGCCACCCCGTATACCCGCTACCATCCCATGGACTACTACTGGTGGCTTCGGATGCA GATCATGACCCATTTTCCTGGAGCCATCTCTGACAAGATCTACATACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205162 representing BC096457
Red=Cloning site Green=Tags(s) MLAARLSRPLSQLPGKALSVRDRENGTRHTLLFYPASFSPDTRRTYASQADAASGKAILITGCDSGFGFS LAKHLHSKGFLVFAGCLMKDKGDAGVKELDSLKSDRLRTIQLNVCNSEEVEKAVETIRSGLKDPEKGMWG LVNNAGISTFGEVEFTSMETYKEVAEVNLWGTVRTTKSFLPLLRRAKGRVVNISSMLGRMANPARSPYCI TKFGVEAFSDCLRYEMHPLGVKVSVVEPGNFIAATSLYSPERIQAIAKKMWDDLPEVVRKDYGRKYFDEK IAKMETYCNSGSTDTSSVINAVTHALTAATPYTRYHPMDYYWWLRMQIMTHFPGAISDKIYIH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC096457 |
ORF Size | 1031 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC096457, AAH96457 |
RefSeq Size | 1657 bp |
RefSeq ORF | 1031 bp |
Locus ID | 71911 |
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