Noxo1 (BC019525) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Snx28, P41NOX, P41NOXA, P41NOXB, P41NOXC
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205276 representing BC019525 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCCCAAGACACCCAGTATCAGCCCATGCTGTAGCCTTGGTGCAAATGGACCGACTCCAGACAT TTGCCTTCTCCGTGTGCTGGTCAGACAACAGTGACACATTTGTACGGAGGAGCTGGGATGAGTTCAGGCA GCTCCAGAAGACCCTTAAGAAAACCTTCCCAGTGGAGGCAGGCCTGCTACGGAGATCTGAACAAGTTCTT CCCAAGCTTCCTGATGCTCCATTGCTGACACGTCGGGGGCATACTGGTCGAGGACTGGTACGTTTGCGGC TGCTGGACACCTATGTACAGGCATTGCTGGCAACCTCAGAACACATATTGAGGAGTTCAGCACTTCACGG CTTCTTTGTACCCAAACCTCTGGATCTGGAGCCCATGCTGCCTCCTGGCAGCCTGGTGATCCTGCCTACA CCAGAGGAGCCCTTATCCCAACCCAGAGGCAGCCTTGACATTCACAGCCTGGAGGCTCAGAGCATTCCCT GTGTACAGCCTTTCCACACTCTTGACATAAGAGACAGACCTTTCCACACCAAGGCTCAAGAAATTCTGGA CATATTACTACGACATCCTTCAGGCTGGTGGCTGGTGGAGAACAAGGATCAGCAGGTAGCCTGGTTTCCA GCTCCCTACCTGGAGGAGGTAGCAACGTGCCAAGGCCAGGAGTCAGGCCTGGCTTTGCAAGGAAGTGGGA GGCAGTTCTGCACTACCCAGGCCTACGAGGGCAGTCGCTCTGATGAGCTATCCGTGCCCTCAGGGGCACG TGTCCATGTGCTGGAGACCTCAGACCGAGGCTGGTGGCTGTGCAGGTATAATGGCCGGACAGGCCTACTC CCTGCAATGTCGCTGCAACCTGAAGGGCTGGGCTCACTCCTGGGCAGGCCAGGGTTCCCAGACAGTGCTG GGGCAGACAAGGTGGCTGAGGACAGAACCATTCCCCCTGTAGTACCAACTCGTCCCTGTATGAGTGCCAT CCAGAGTCGATGCTGCTCCATTACCCGCAGGGCACTGGCACAGGAACAAGGGACTCGGGTTCCCCGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205276 representing BC019525 Red=Cloning site Green=Tags(s) MASPRHPVSAHAVALVQMDRLQTFAFSVCWSDNSDTFVRRSWDEFRQLQKTLKKTFPVEAGLLRRSEQVL PKLPDAPLLTRRGHTGRGLVRLRLLDTYVQALLATSEHILRSSALHGFFVPKPLDLEPMLPPGSLVILPT PEEPLSQPRGSLDIHSLEAQSIPCVQPFHTLDIRDRPFHTKAQEILDILLRHPSGWWLVENKDQQVAWFP APYLEEVATCQGQESGLALQGSGRQFCTTQAYEGSRSDELSVPSGARVHVLETSDRGWWLCRYNGRTGLL PAMSLQPEGLGSLLGRPGFPDSAGADKVAEDRTIPPVVPTRPCMSAIQSRCCSITRRALAQEQGTRVPR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC019525
ORF Size	1049 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019525, AAH19525
RefSeq Size	1260 bp
RefSeq ORF	1049 bp
Locus ID	71893
Gene Summary	Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.[UniProtKB/Swiss-Prot Function]

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