Sord (NM_146126) Mouse Tagged ORF Clone
CAT#: MG205452
- TrueORF®
Sord (tGFP-tagged) - Mouse sorbitol dehydrogenase (Sord)
ORF Plasmid: DDK
"NM_146126" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Sdh-1; Sdh1; Sodh-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205452 representing NM_146126
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTCCAGCTAAGGGCGAGAACCTGTCCCTGGTGGTGCACGGACCTGGAGACATCCGCCTGGAGA ACTACCCAATACCTGAGCTCGGCCCAAATGATGTGTTACTAAAGATGCACTCGGTGGGGATTTGCGGCTC AGATGTTCACTACTGGGAGCATGGACGAATTGGGGACTTTGTTGTGAAAAAGCCAATGGTGCTTGGGCAT GAGGCTGCTGGAACAGTCACAAAAGTAGGAGAGTTGGTGAAACATCTGAAACCAGGAGATCGGGTTGCCA TCGAGCCTGGCGTTCCCCGAGAAGTAGATGAGTACTGCAAGATTGGCCGGTACAACCTGACGCCGACTAT CTTCTTCTGTGCCACGCCCCCGGATGATGGAAACCTCTGCCGATTCTACAAGCACAATGCTGACTTCTGC TACAAGCTTCCTGACAGTGTCACTTTTGAAGAAGGGGCCCTGATTGAACCTCTCTCTGTGGGGATCTATG CCTGCCGTCGAGGTTCAGTTTCCCTGGGGAACAAGGTCCTTGTGTGTGGCGCTGGGCCAGTTGGGATGGT CACTTTGCTTGTGGCCAAAGCAATGGGAGCTGCTCAAGTTGTGGTGACTGACCTATCCGCTTCTCGGTTG ACCAAAGCCAAGGAAGTTGGAGCAGACTTTACCATCCAGGTTGGCAAAGAGACCCCTCAGGAAATTGCCA GTAAGGTGGAAAGCCTGCTGGGAAGCAAGCCGGAGGTTACCATTGAGTGCACAGGAGCCGAGTCCTCCGT CCAGACGGGCATCTATGCCACTCACTCTGGTGGGACCTTGGTGATTGTGGGAATGGGCGCCGAGATGGTC AATTTACCCCTGGTGCACGCAGCCATTCGGGAAGTGGATATCAAAGGCGTGTTTCGATACTGCAACACGT GGCCGATGGCAATTTCCATGCTTGCATCGAAGACGTTGAATGTAAAGCCCTTAGTTACCCACAGATTCCC CCTGGAGAAGGCAGTTGAAGCCTTTGAAACAGCCAAAAAGGGAGTGGGGCTGAAAGTTATGATCAAGTGT GACCCCAATGACCAAAACCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205452 representing NM_146126
Red=Cloning site Green=Tags(s) MAAPAKGENLSLVVHGPGDIRLENYPIPELGPNDVLLKMHSVGICGSDVHYWEHGRIGDFVVKKPMVLGH EAAGTVTKVGELVKHLKPGDRVAIEPGVPREVDEYCKIGRYNLTPTIFFCATPPDDGNLCRFYKHNADFC YKLPDSVTFEEGALIEPLSVGIYACRRGSVSLGNKVLVCGAGPVGMVTLLVAKAMGAAQVVVTDLSASRL TKAKEVGADFTIQVGKETPQEIASKVESLLGSKPEVTIECTGAESSVQTGIYATHSGGTLVIVGMGAEMV NLPLVHAAIREVDIKGVFRYCNTWPMAISMLASKTLNVKPLVTHRFPLEKAVEAFETAKKGVGLKVMIKC DPNDQNP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_146126 |
ORF Size | 1071 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146126.4 |
RefSeq Size | 2260 bp |
RefSeq ORF | 1074 bp |
Locus ID | 20322 |
UniProt ID | Q64442 |
Gene Summary | Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols (By similarity). Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose (PubMed:6852349). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility and protein tyrosine phosphorylation (PubMed:18799757). Has no activity on ethanol. Cannot use NADP(+) as the electron acceptor (PubMed:6852349).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204150 | Sord (untagged) - Mouse sorbitol dehydrogenase (Sord), (10ug) |
CNY 3,656.00 |
|
MR205452 | Sord (Myc-DDK-tagged) - Mouse sorbitol dehydrogenase (Sord) |
CNY 3,656.00 |
|
MR205452L3 | Lenti ORF clone of Sord (Myc-DDK-tagged) - Mouse sorbitol dehydrogenase (Sord) |
CNY 5,230.00 |
|
MR205452L4 | Lenti ORF clone of Sord (mGFP-tagged) - Mouse sorbitol dehydrogenase (Sord) |
CNY 5,230.00 |