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Wnt9a (BC066165) Mouse Tagged ORF Clone
CAT＃: MG205524
- TrueORF®
Wnt9a (tGFP-tagged) - Mouse wingless-type MMTV integration site 9A (cDNA clone MGC:76532 IMAGE:30435371)
ORF Plasmid: DDK
"BC066165" in other vectors (4)
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CNY 3,990.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Wnt14 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205524 representing BC066165
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATGGGTCCCTTCTGGCGCGCTGGCTGGCCGCGGCCTTCGGGCTGACGCTGCTGCTCGCCGCGC TGCGCCCTTCGGCCGCCTACTTCGGGCTAACAGGCAGTGAACCCCTGACTATCCTCCCTCTGACCCTGGA GACCGAGGCTGCGGCCCAAGCACACTACAAGGCCTGCGACAGGCTGAAGCTGGAGCGCAAGCAGCGCCGC ATGTGCCGCAGGGACCCGGGTGTGGCCGAGACACTGGTGGAGGCCGTAAGCATGAGTGCCCTGGAGTGCC AGTACCAGTTCCGCTTTGAGCGCTGGAACTGCACCCTGGAGGGCCGCTACCGAGCCAGCCTGCTCAAGCG AGGCTTCAAGGAGACTGCTTTCCTCTACGCCATCTCTTCTGCCGGCCTGACGCATGCACTGGCCAAGGCC TGCAGTGCAGGCCGCATGGAGCGCTGCACGTGTGATGAGGCACCCGACCTGGAAAACCGCGAGGCCTGGC AGTGGGGCGGCTGCGGGGACAACCTCAAGTACAGCAGCAAGTTTGTCAAGGAGTTCCTGGGCCGGCGCTC TAGCAAGGATTTGCGAGCCCGAGTGGACTTCCACAACAACCTCGTGATAAAGGCTGGAGTGGAAACCACT TGCAAATGCCATGGTGCGTCTGGCTCCTGCACCGTGCGGACCTGCTGGCGGCAGCTAGCACCCTTCCACG AGGTGGGCAAGCACCTAAAACACAAATATGAGACCTCGCTCAAGGTGGGCAGCACTACCAATGAAGCCAC TGGAGAGGCAGGTGCCATCTCCCCACCGCGGGGCCGGGCTTCTGGGTCAGGAGGTGGCGACCCACTGCCC CGAACACCAGAGCTTGTACACCTGGACGACTCTCCCAGCTTCTGCCTGGCTGGCCGCTTTTCCCCTGGCA CGGCAGGCCGCAGGTGTCACCGGGAGAAGAACTGTGAGAGTATTTGTTGTGGCCGAGGCCACAACACACA GAGTCGTGTGGTGACAAGGCCCTGCCAATGCCAGGTCCGCTGGTGCTGCTACGTGGAGTGCAGGCAGTGT ATACAGAGAGAGGAGGTCTATACCTGCAAGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205524 representing BC066165
Red=Cloning site Green=Tags(s) MLDGSLLARWLAAAFGLTLLLAALRPSAAYFGLTGSEPLTILPLTLETEAAAQAHYKACDRLKLERKQRR MCRRDPGVAETLVEAVSMSALECQYQFRFERWNCTLEGRYRASLLKRGFKETAFLYAISSAGLTHALAKA CSAGRMERCTCDEAPDLENREAWQWGGCGDNLKYSSKFVKEFLGRRSSKDLRARVDFHNNLVIKAGVETT CKCHGASGSCTVRTCWRQLAPFHEVGKHLKHKYETSLKVGSTTNEATGEAGAISPPRGRASGSGGGDPLP RTPELVHLDDSPSFCLAGRFSPGTAGRRCHREKNCESICCGRGHNTQSRVVTRPCQCQVRWCCYVECRQC IQREEVYTCKG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC066165 |
| ORF Size | 1083 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066165, AAH66165 |
| RefSeq Size | 3306 bp |
| RefSeq ORF | 1085 bp |
| Locus ID | 216795 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development (PubMed:16818445). Plays a redundant role in maintaining joint integrity (PubMed:16818445).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218580 | Wnt9a (untagged) - Mouse wingless-type MMTV integration site 9A (cDNA clone MGC:76532 IMAGE:30435371), (10ug) |
CNY 6,940.00 |
|
| MR205524 | Wnt9a (Myc-DDK-tagged) - Mouse wingless-type MMTV integration site 9A (cDNA clone MGC:76532 IMAGE:30435371) |
CNY 3,610.00 |
|
| MR205524L3 | Lenti ORF clone of Wnt9a (Myc-DDK-tagged) - Mouse wingless-type MMTV integration site 9A (cDNA clone MGC:76532 IMAGE:30435371) |
CNY 5,510.00 |
|
| MR205524L4 | Lenti ORF clone of Wnt9a (mGFP-tagged) - Mouse wingless-type MMTV integration site 9A (cDNA clone MGC:76532 IMAGE:30435371) |
CNY 5,510.00 |
