Slc10a1 (BC021154) Mouse Tagged ORF Clone

CAT#: MG205550

  • TrueORF®

Slc10a1 (tGFP-tagged) - Mouse solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (cDNA clone MGC:13910



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


SLC10A1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Ntcp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205550 representing BC021154
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCACAACGTATCAGCCCCCTTCAATTTCTCCCTGCCGCCTGGCTTTGGCCACCGGGCCACAG
ACACTGCGCTCAGCGTCATTCTGGTAGTTATGTTGCTGCTCATCATGCTCTCGCTTGGCTGCACCATGGA
GTTCAGCAAGATCAAGGCTCACTTCTGGAAGCCCAAAGGGGTGATCATCGCCATAGTGGCCCAGTACGGT
ATCATGCCCCTCAGTGCTTTCCTTCTGGGCAAGGTCTTTCATCTGACCAGCATTGAGGCTCTGGCCATCC
TCATCTGCGGCTGCTCTCCTGGGGGGAACCTGTCTAACCTCTTCACCCTGGCCATGAAGGGGGACATGAA
CCTCAGCATTGTGATGACCACCTGCTCCAGCTTCACTGCCTTGGGCATGATGCCTCTCCTCTTATACATC
TACAGCAAAGGAATCTACGACGGAGATCTTAAGGACAAGGTGCCCTACAAAGGCATTATGTTATCACTCG
TCATGGTTCTCATTCCTTGCGCCATAGGGATCTTCCTGAAGTCCAAAAGGCCACACTATGTACCCTACGT
CCTCAAGGCAGGCATGATCATCACTTTCTCCCTCTCTGTGGCTGTCACAGTCCTGTCTGTCATCAATGTG
GGCAACAGCATCATGTTCGTCATGACACCACACTTACTGGCTACCTCCTCCCTGATGCCTTTCACTGGCT
TCCTGATGGGCTACATTCTCTCTGCTCTCTTCCGACTAAATCCAAGCTGCAGACGCACCATCAGCATGGA
AACAGGATTCCAAAACGTCCAACTCTGTTCTACCATCCTCAATGTCACCTTCCCCCCTGAAGTCATTGGA
CCACTGTTCTTCTTTCCTCTCCTTTATATGATTTTTCAGCTTGCAGAAGGACTTCTCTTCATTATTATCT
TCCGGTGCTATTTGAAAATCAAACCTCAGAAGGACCAAACAAAAATTACCTACAAGGCTGCTGCAACAGA
AGATGCTACTCCAGCAGCTCTGGAAAAAGGTACCCACAACGGGAATAATCCTCCTACACAACCTGGCCTT
TCCCCTAATGGCCTGAACTCTGGTCAGATGGCAAAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205550 representing BC021154
Red=Cloning site Green=Tags(s)

MEAHNVSAPFNFSLPPGFGHRATDTALSVILVVMLLLIMLSLGCTMEFSKIKAHFWKPKGVIIAIVAQYG
IMPLSAFLLGKVFHLTSIEALAILICGCSPGGNLSNLFTLAMKGDMNLSIVMTTCSSFTALGMMPLLLYI
YSKGIYDGDLKDKVPYKGIMLSLVMVLIPCAIGIFLKSKRPHYVPYVLKAGMIITFSLSVAVTVLSVINV
GNSIMFVMTPHLLATSSLMPFTGFLMGYILSALFRLNPSCRRTISMETGFQNVQLCSTILNVTFPPEVIG
PLFFFPLLYMIFQLAEGLLFIIIFRCYLKIKPQKDQTKITYKAAATEDATPAALEKGTHNGNNPPTQPGL
SPNGLNSGQMAN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC021154
ORF Size 1088 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021154, AAH21154
RefSeq Size 1649 bp
RefSeq ORF 1088 bp
Locus ID 20493
Gene Summary The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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