Tarbp2 (BC002028) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Prbp; TRBP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205621 representing BC002028 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAGGATCAGGGCTCCGGCACTACTACAGGCTGCGGGCTGCCCAGCATAGAGCAAATGCTGG CCGCCAACCCGGGCAAGACCCCGATCAGCCTTCTTCAGGAGTATGGGACCAGAATAGGAAAGACGCCCGT GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCATCAACCTAATTTCACCTTTCGGGTCACCGTTGGCGAC ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC ACCTCAAAGGGGGGAGCATGCTGGAACCAGCCCTGGAGGACAGCAGTTCTTTTTCTCTCCTAGACTCTTC ACCGCCTGAGGACACTCCTGTCGTTGCTGCAGAAGCTGCTGCCCCTGTTCCATCTGCTGTACTAACCAGG AGCCCTCCCATGGAGATGCAGCCCCCTGTCTCTCCTCAGCAGTCTGAGTGCAACCCCGTCGGTGCTCTGC AGGAGCTGGTGGTGCAAAAAGGCTGGCGTTTGCCAGAGTACATGGTGACCCAAGAGTCTGGGCCTGCTCA CCGCAAAGAGTTCACCATGACTTGCCGGGTGGAGCGTTTCATTGAGATTGGCAGTGGCACTTCCAAAAAG CTGGCAAAGCGTAACGCAGCAGCTAAGATGCTCCTTCGAGTGCACACTGTACCTCTGGATGCCCGGGATG GCAATGAGGCAGAGCCTGATGACGATCATTTTTCCATTGGCGTGAGCTCCCGCCTGGATGGACTGAGGAA TCGTGGGCCAGGCTGCACCTGGGATTCCTTGCGGAATTCTGTGGGAGAAAAGATCCTATCCCTTCGCAGT TGCTCCGTGGGCTCTCTAGGGGCTCTGGGCTCTGCCTGCTGCAGTGTCCTCAGTGAGCTCTCTGAGGAGC AGGCTTTCCATGTCAGCTATCTGGATATTGAGGAACTGAGCCTGAGTGGGCTCTGCCAGTGCCTAGTGGA ACTGTCCACCCAGCCAGCCACTGTGTGTTATGGTTCTGCAACCACCAGGGAGGCAGCCCGAGGTGATGCT GCTCACCGCGCCCTACAGTACCTCAGGATCATGGCGGGTAGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205621 representing BC002028 Red=Cloning site Green=Tags(s) MSEEDQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGD TSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSLLDSSPPEDTPVVAAEAAAPVPSAVLTR SPPMEMQPPVSPQQSECNPVGALQELVVQKGWRLPEYMVTQESGPAHRKEFTMTCRVERFIEIGSGTSKK LAKRNAAAKMLLRVHTVPLDARDGNEAEPDDDHFSIGVSSRLDGLRNRGPGCTWDSLRNSVGEKILSLRS CSVGSLGALGSACCSVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCYGSATTREAARGDA AHRALQYLRIMAGSK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002028
ORF Size	1097 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002028, AAH02028
RefSeq Size	1514 bp
RefSeq ORF	1097 bp
Locus ID	21357
Gene Summary	Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity). Binds in vitro to the PRM1 3' UTR. Seems to act as a repressor of translation (PubMed:8649414).[UniProtKB/Swiss-Prot Function]

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