Tarbp2 (BC002028) Mouse Tagged ORF Clone
CAT#: MG205621
- TrueORF®
Tarbp2 (tGFP-tagged) - Mouse TAR (HIV) RNA binding protein 2 (cDNA clone MGC:5911 IMAGE:3489376)
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CNY 3,710.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Prbp; TRBP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205621 representing BC002028
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAGGATCAGGGCTCCGGCACTACTACAGGCTGCGGGCTGCCCAGCATAGAGCAAATGCTGG CCGCCAACCCGGGCAAGACCCCGATCAGCCTTCTTCAGGAGTATGGGACCAGAATAGGAAAGACGCCCGT GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCATCAACCTAATTTCACCTTTCGGGTCACCGTTGGCGAC ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC ACCTCAAAGGGGGGAGCATGCTGGAACCAGCCCTGGAGGACAGCAGTTCTTTTTCTCTCCTAGACTCTTC ACCGCCTGAGGACACTCCTGTCGTTGCTGCAGAAGCTGCTGCCCCTGTTCCATCTGCTGTACTAACCAGG AGCCCTCCCATGGAGATGCAGCCCCCTGTCTCTCCTCAGCAGTCTGAGTGCAACCCCGTCGGTGCTCTGC AGGAGCTGGTGGTGCAAAAAGGCTGGCGTTTGCCAGAGTACATGGTGACCCAAGAGTCTGGGCCTGCTCA CCGCAAAGAGTTCACCATGACTTGCCGGGTGGAGCGTTTCATTGAGATTGGCAGTGGCACTTCCAAAAAG CTGGCAAAGCGTAACGCAGCAGCTAAGATGCTCCTTCGAGTGCACACTGTACCTCTGGATGCCCGGGATG GCAATGAGGCAGAGCCTGATGACGATCATTTTTCCATTGGCGTGAGCTCCCGCCTGGATGGACTGAGGAA TCGTGGGCCAGGCTGCACCTGGGATTCCTTGCGGAATTCTGTGGGAGAAAAGATCCTATCCCTTCGCAGT TGCTCCGTGGGCTCTCTAGGGGCTCTGGGCTCTGCCTGCTGCAGTGTCCTCAGTGAGCTCTCTGAGGAGC AGGCTTTCCATGTCAGCTATCTGGATATTGAGGAACTGAGCCTGAGTGGGCTCTGCCAGTGCCTAGTGGA ACTGTCCACCCAGCCAGCCACTGTGTGTTATGGTTCTGCAACCACCAGGGAGGCAGCCCGAGGTGATGCT GCTCACCGCGCCCTACAGTACCTCAGGATCATGGCGGGTAGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205621 representing BC002028
Red=Cloning site Green=Tags(s) MSEEDQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGD TSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSLLDSSPPEDTPVVAAEAAAPVPSAVLTR SPPMEMQPPVSPQQSECNPVGALQELVVQKGWRLPEYMVTQESGPAHRKEFTMTCRVERFIEIGSGTSKK LAKRNAAAKMLLRVHTVPLDARDGNEAEPDDDHFSIGVSSRLDGLRNRGPGCTWDSLRNSVGEKILSLRS CSVGSLGALGSACCSVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCYGSATTREAARGDA AHRALQYLRIMAGSK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC002028 |
ORF Size | 1097 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC002028, AAH02028 |
RefSeq Size | 1514 bp |
RefSeq ORF | 1097 bp |
Locus ID | 21357 |
Gene Summary | Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity). Binds in vitro to the PRM1 3' UTR. Seems to act as a repressor of translation (PubMed:8649414).[UniProtKB/Swiss-Prot Function] |
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