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Ctns (NM_031251) Mouse Tagged ORF Clone
CAT＃: MG205647
- TrueORF®
Ctns (tGFP-tagged) - Mouse cystinosis, nephropathic (Ctns)
ORF Plasmid: DDK
"NM_031251" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI195360; AW049661 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205647 representing NM_031251
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGAATTGGCTGCTTATTTTGACCCTTTTTCTCTTGATGTTCATAGAGAAGTATGAGTCAACTG TCAGTCTCACTGCTCCTCCCACCGTGAAGCTGGAAAATGGCAGTTCAACCAACGTCGACATCACCCTTGG GCATCCACTAAATTCGACCTTGGTGATCACTTTTGAAGTCACGTTTCGCTCAAAAAATCTGACTATTGTG GAGCTTCCTGATGAAGTTATAGTGCCTCGCGGAGAGAAAAATGCCTCTTTCCAAGTGACTTCTCAAAATA TTGGACAAGTGACTGTTTTTCTGCATGGAAATCATTCCAACCAGACCTGCCCCAGGATCCGGTTCTTGGT GATACACAGCAGAATCGTTAGCATCATAAACCAGGTGATTGGCTGGATCTACTTCATGGCGTGGTCTGTC TCCTTTTACCCACAAGTGATCCAGAACTGGAGACGGAAAAGTGTCATTGGTCTCAGCTTTGATTTCTTGG CCCTGAACTTGACGGGCTTCGTGGCCTACAGTGTTTTCAACATCGGTCTTTTGTGGGTGCCCTATATCCA GGAGGAGTTTCTCCTCAAATACCCCAATGGTGTGAACCCCGTCGACAGTAATGATGCCTTCTTCAGCTTG CATGCTGTCGCCCTCACCCTGATTGTCATCCTGCAGTGCTGCCTGTATGAGCGAGGCAACCAGCGTGTGT CATGGCCCTCCATTGGCTTCCTGGTACTCGCATGGCTCTTTGTCCTAGTCACCATGATTGTGGCTGCAGT CGGTATCACCACATGGCTCCAGTTCCTCTTCTGCTTCTCCTACATCAAGCTCATCATCACACTGATCAAG TATTTCCCACAGGCCTATATGAACTTCTACTACAAAAGCACCAAGGGCTGGAGCATTGGCGGTGTGCTCC TCGATTTTACAGGGGGCAGCTTTAGCCTCCTCCAGATGTTCCTCCAGTCTTACAATAATGACCAATGGAC ATTGATCTTCGGAGACCCAACCAAGTTCGGACTTGGCGTCTTCACCATCTTCTTTGATGTTGTCTTCTTC ATCCAGCACTTCTACTTGTACAGAAAGAAACCAGGGTACGACCAGTTGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205647 representing NM_031251
Red=Cloning site Green=Tags(s) MRRNWLLILTLFLLMFIEKYESTVSLTAPPTVKLENGSSTNVDITLGHPLNSTLVITFEVTFRSKNLTIV ELPDEVIVPRGEKNASFQVTSQNIGQVTVFLHGNHSNQTCPRIRFLVIHSRIVSIINQVIGWIYFMAWSV SFYPQVIQNWRRKSVIGLSFDFLALNLTGFVAYSVFNIGLLWVPYIQEEFLLKYPNGVNPVDSNDAFFSL HAVALTLIVILQCCLYERGNQRVSWPSIGFLVLAWLFVLVTMIVAAVGITTWLQFLFCFSYIKLIITLIK YFPQAYMNFYYKSTKGWSIGGVLLDFTGGSFSLLQMFLQSYNNDQWTLIFGDPTKFGLGVFTIFFDVVFF IQHFYLYRKKPGYDQLN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031251 |
| ORF Size | 3007 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031251.4, NP_112541.1 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 83429 |
| UniProt ID | P57757 |
| Gene Summary | Cystine/H(+) symporter thought to transport cystine out of lysosomes. Plays an important role in melanin synthesis, possibly by preventing melanosome acidification and subsequent degradation of tyrosinase TYR.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203663 | Ctns (untagged) - Mouse cystinosis, nephropathic (Ctns), (10ug) |
CNY 2,000.00 |
|
| MR205647 | Ctns (Myc-DDK-tagged) - Mouse cystinosis, nephropathic (Ctns) |
CNY 2,850.00 |
|
| MR205647L3 | Lenti ORF clone of Ctns (Myc-DDK-tagged) - Mouse cystinosis, nephropathic (Ctns) |
CNY 4,750.00 |
|
| MR205647L4 | Lenti ORF clone of Ctns (mGFP-tagged) - Mouse cystinosis, nephropathic (Ctns) |
CNY 4,750.00 |
