Popdc2 (BC064005) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AV006127; Pop2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205720 representing BC064005 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCAATGGCAGCTCGGTGGCTCAGCTCCTCTGGCAGCCGCCCGTGTGCAGGAGCTGGAAACCGG ATGTAGAAGGAGCTGTCTATCACCTGGCCAACTGTTTCTTGCTTATGGGCTTTATGGCAGGCAGTGGAGT GTATGGATGCTTCTATCTTTTCGGCATCCTGGGCCCAGGCTACCTCTGCTGCGTGCTGTGGGGCTGGTTT GATGCTTGTGGACTAGACATCGTCCTTTGGAACGTCCTCCTGACAGTGGCTTGCCTGCTTCAGCTGGCAC AGCTGGTCTATCGTGTCCGGGTGAATACCCTCCCGGAGGAATTCAATCTCCTCTACAGGACACTGTGCCT GCCCCTGCAGGTGCCCCTGCAGGTCTACAAGGAGATTGTCCACTGCTGCCATGAGCAGGTCTTGACGCTG GCCACAGAGCAGACCTACGCTGTGGAGGGGGAGACCCCCATCAATCGCCTGTCCCTGCTCCTCTCAGGCC GGGTTCGAGTGAGCCAAGACGGGCAGTTTCTGCACTACATCTTTCCGTATCAGTTCATGGACTCTCCTGA ATGGGAATCACTGCATCCTTCTGAAGAGGGGACCTTCCAGGTGACGCTGACTGCGGAGACCGAATGTAGC TACATTTCCTGGCCCCGGAAAAATCTTTACCTTCTTCTGAACAGAGAGCGATACATCTCCCGCCTCTTCT CAGCCCTGCTGGGCTATGACATCTCGGAGAAGCTCTATACCCTCAATGACAAGCTGTTTGCCAAGTTTGG GTTACGCTTCGACATCCGCCTTCCCAGCCTCTACCACGTTCTGAGTCCCTCTGCCTCAGACGGGGAACCA GAGTCTGAGAAAGATGATGAAGAAGCCCTTGAGGCAGCTGTGTCCCCTGCTCAGGCCAGGCCCATCTGCA TCGTGCCAACACCCCCTTGTTCCGCGCCTCCAGCAACCACCAACTTTCCCGTGCCTCTGCCCCGGGCCAG GATGCCCAGGATGCCCAGGCCGGACAGCGGCAACCTGGGTGAGGACTCCACCAGTCTGGTGCTGGAGGAT TTTGAGGAGGTTTCAGGATCAGAGTCGTTTATGGATTATAGGAGCGATGGGGAGTACATGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205720 representing BC064005 Red=Cloning site Green=Tags(s) MSANGSSVAQLLWQPPVCRSWKPDVEGAVYHLANCFLLMGFMAGSGVYGCFYLFGILGPGYLCCVLWGWF DACGLDIVLWNVLLTVACLLQLAQLVYRVRVNTLPEEFNLLYRTLCLPLQVPLQVYKEIVHCCHEQVLTL ATEQTYAVEGETPINRLSLLLSGRVRVSQDGQFLHYIFPYQFMDSPEWESLHPSEEGTFQVTLTAETECS YISWPRKNLYLLLNRERYISRLFSALLGYDISEKLYTLNDKLFAKFGLRFDIRLPSLYHVLSPSASDGEP ESEKDDEEALEAAVSPAQARPICIVPTPPCSAPPATTNFPVPLPRARMPRMPRPDSGNLGEDSTSLVLED FEEVSGSESFMDYRSDGEYMR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC064005
ORF Size	1115 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064005, AAH64005
RefSeq Size	2184 bp
RefSeq ORF	1115 bp
Locus ID	64082
Gene Summary	This gene encodes a member of the Popeye domain containing family of membrane proteins. Proteins of this family contain three helical transmembrane domains and a conserved intracellular Popeye domain. In the adult mouse, this gene is expressed at high levels in cardiac myocytes, and mice deficient for this gene develop stress-induced cardiac pacemaker dysfunction. The protein binds to a two-pore domain potassium channel and recruits it to the plasma membrane. Cyclic adenosine monophosphate negatively regulates this interaction through the Popeye domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

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