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Nsfl1c (NM_198326) Mouse Tagged ORF Clone
CAT＃: MG205743
- TrueORF®
Nsfl1c (tGFP-tagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c)
ORF Plasmid: DDK
"NM_198326" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Munc-18c; p47; Stxbp3a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205743 representing NM_198326
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGAGCGGCAGGACGCGTTGAGGGAGTTCGTGGCGGTGACTGGCACTGAGGAGGACAGGGCCC GTTTCTTCCTGGAGTCGGCTGGCTGGGACCTGCAGATTGCACTTGCAAGCTTTTATGAGGATGGAGGGGA TGAAGACATTGTGACCATTTCACAAGCAACCCCAAGTTCAGTGTCCAGAGGCACAGCTCCTAGTGATAAT AGAGTGACATCCTTCAGAGACCTCATTCATGACCAAGATGAAGAGGAGGAGGAAGAGGAGGGACAGAGGA GCAGGTTTTATGCTGGGGGCTCAGAGAGAAGTGGACAGCAGATTGTTGGTCCTCCCCGGAAGAAAAGTCC CAACGAGCTGGTGGATGATCTGTTTAAGGGTGCCAAAGAGCATGGGGCTGTAGCTGTGGAACGAGTGACC AAGAGCCCTGGAGAGACCAGTAAACCGAGACCATTTGCAGGAGGGGGCTACCGCCTTGGAGCAGCACCAG AGGAAGAGTCTGCCTATGTGGCAGGAGAAAGGAGGCGGCATTCAGGCCAGGATGTTCATGTAGTGCTAAA GCTCTGGAAAACTGGATTCAGCCTAGACAATGGTGACCTTAGAAGCTACCAAGACCCATCCAATGCCCAG TTTCTGGAGTCTATCCGTAGAGGGGAGGTTCCTGCAGAGCTTCGGAGGTTAGCGCATGGTGGGCAGGTGA ACCTGGATATGGAGGATCATCGGGATGAGGACTTCGTGAAGCCCAAGGGAGCTTTCAAAGCCTTCACTGG CGAGGGCCAGAAACTGGGCAGCACGGCCCCCCAGGTATTAAACACCAGCTCTCCAGCCCAACAAGCAGAA AATGAAGCCAAAGCTAGCTCGTCCATCTTAATCAATGAAGCAGAACCTACCACGAACATCCAAATCCGGC TTGCAGATGGCGGGAGGCTGGTGCAGAAATTCAACCACAGCCACAGGATCAGCGACATCCGGCTCTTCAT CGTGGATGCCCGGCCAGCTATGGCTGCCACCAGTTTTGTCCTTATGACTACCTTCCCTAACAAAGAGCTG GCTGATGAGAACCAAACCCTGAAGGAAGCCAACCTTCTCAACGCTGTCATCGTGCAGCGGTTAACA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205743 representing NM_198326
Red=Cloning site Green=Tags(s) MAEERQDALREFVAVTGTEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDN RVTSFRDLIHDQDEEEEEEEGQRSRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVT KSPGETSKPRPFAGGGYRLGAAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQ FLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPAQQAE NEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKEL ADENQTLKEANLLNAVIVQRLT SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198326 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198326.3 |
| RefSeq Size | 1355 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 386649 |
| UniProt ID | Q9CZ44 |
| Gene Summary | Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202643 | Nsfl1c (untagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c), (10ug) |
CNY 3,656.00 |
|
| MR205743 | Nsfl1c (Myc-DDK-tagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c) |
CNY 3,656.00 |
|
| MR205743L3 | Lenti ORF clone of Nsfl1c (Myc-DDK-tagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c) |
CNY 5,230.00 |
|
| MR205743L4 | Lenti ORF clone of Nsfl1c (mGFP-tagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c) |
CNY 5,230.00 |
