Cntfr (BC050928) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Cntfralpha
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205753 representing BC050928 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTTCTGTCCCATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGA AACACAGTCCACAGGAGGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGG GACAGCGAGCTGGGACGCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAAC GGCTCTCAGCTGATACTGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACT CCTGGCACCTGCGCCACCAAGTCCTTCTACATGTGGGTTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCG CTCCAACACTTACCCCAAGGGCTTCTACTGCAGCTGGCACCTGCCCACCCCCACCTACATCCCCAATACC TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCT GCCACATTCGGTACATGCACCTGTTCTCAACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAACGCCTT GGGTCACAACACTACGGCCATCACCTTTGACGAATTCACCATTGTGAAGCCTGATCCTCCAGAAAACGTG GTGGCCCGGCCAGTGCCCAGCAACCCCCGTCGGCTGGAGGTGACATGGCAGACACCCTCAACTTGGCCCG ACCCTGAATCCTTTCCTCTCAAGTTCTTCCTGCGCTACCGGCCTCTCATCCTGGACCAATGGCAGCATGT GGAGCTCTCGGATGGCACAGCACACACCATCACAGATGCCTACGCAGGGAAGGAATACATCATCCAGGTG GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTGGCTGCTCACGCCACACCCTGGACTG AGGAACCTCGGCATCTCACAACAGAAGCCCAGGCCCCCGAGACCACGACCAGCACCACCAGCTCATTGGC ACCCCCACCCACCACGAAGATCTGTGACCCTGGGGAGCTTGGCAGCGGCGGAGGACCCTCCATACTCTTC TTGACCAGTGTCCCAGTCACTCTGGTCCTGGCTGCCGCTGCTGCCACAGCCAACAATCTCCTGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205753 representing BC050928 Red=Cloning site Green=Tags(s) MAASVPWACCAVLAAAAAAVYTQKHSPQEAPHVQYERLGADVTLPCGTASWDAAVTWRVNGTDLAPDLLN GSQLILRSLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNT FNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNTTAITFDEFTIVKPDPPENV VARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQV AAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAPETTTSTTSSLAPPPTTKICDPGELGSGGGPSILF LTSVPVTLVLAAAAATANNLLI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050928
ORF Size	1118 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050928, AAH50928
RefSeq Size	2017 bp
RefSeq ORF	1118 bp
Locus ID	12804
Gene Summary	This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]

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