Glul (BC015086) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Glns; GS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205788 representing BC015086 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTG AGAAAGTCCAAGCCATGTATATCTGGGTTGATGGTACCGGAGAAGGACTGCGCTGCAAGACCCGTACCCT GGACTGTGAGCCCAAGTGTGTGGAAGAGTTACCTGAGTGGAACTTTGATGGCTCTAGTACCTTTCAGTCT GAAGGCTCCAACAGCGACATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCA ACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAGCCTGCAGAGACCAACTTGAGGCACATCTG TAAACGGATAATGGACATGGTGAGCAACCAGCACCCCTGGTTTGGAATGGAGCAGGAATATACTCTTATG GGAACAGACGGCCACCCATTTGGTTGGCCTTCCAATGGCTTCCCTGGACCCCAAGGCCCGTATTACTGCG GTGTGGGAGCAGACAAGGCCTACGGCAGGGACATCGTGGAGGCTCACTACCGGGCCTGCTTGTATGCTGG AGTCAAGATCACGGGGACAAATGCGGAGGTTATGCCTGCCCAGTGGGAATTCCAGATAGGACCCTGTGAG GGGATCCGAATGGGAGATCATCTTTGGATAGCCCGTTTTATCTTGCATCGGGTGTGCGAAGACTTTGGGG TGATAGCAACCTTTGACCCCAAGCCCATTCCAGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAG CACCAAGGCCATGCGGGAGGAGAATGGTCTGAAGTGCATTGAGGAGGCCATTGACAAACTGAGCAAGAGG CACCAGTACCACATCCGCGCCTACGATCCCAAGGGGGGCCTGGACAACGCCCGGCGTCTGACTGGATTCC ACGAAACCTCCAACATCAACGACTTTTCTGCCGGTGTTGCCAACCGCGGTGCCAGTATCCGCATTCCCCG GACTGTCGGCCAGGAGAAGAAGGGCTACTTTGAAGACCGTCGGCCTTCTGCCAATTGTGACCCCTATGCG GTGACAGAAGCCATCGTCCGCACGTGTCTCCTCAACGAAACAGGCGACGAACCCTTCCAATACAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205788 representing BC015086 Red=Cloning site Green=Tags(s) MATSASSHLNKGIKQMYMSLPQGEKVQAMYIWVDGTGEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQS EGSNSDMYLHPVAMFRDPFRKDPNKLVLCEVFKYNRKPAETNLRHICKRIMDMVSNQHPWFGMEQEYTLM GTDGHPFGWPSNGFPGPQGPYYCGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCE GIRMGDHLWIARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKCIEEAIDKLSKR HQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRGASIRIPRTVGQEKKGYFEDRRPSANCDPYA VTEAIVRTCLLNETGDEPFQYKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC015086
ORF Size	1119 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015086, AAH15086
RefSeq Size	1435 bp
RefSeq ORF	1121 bp
Locus ID	14645
Gene Summary	Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (By similarity). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (PubMed:25870278). Essential for proliferation of fetal skin fibroblasts (By similarity). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development (PubMed:30158707). Involved in angiogenesis by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (By similarity). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (By similarity). Plays a role in ribosomal 40S subunit biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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