Ube2q2 (NM_180600) Mouse Tagged ORF Clone
CAT#: MG205892
- TrueORF®
Ube2q2 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 2 (Ube2q2)
ORF Plasmid: DDK
"NM_180600" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 3010021M21Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205892 representing NM_180600
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAACCACGAGCGAT TCCGCATCGTCAGCTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTGCCGCCGCCGCCACCACCGCC GGGCAGCTCGCTTTCGCCGCCGCCGCCGCTCACACTTCACTGCAACATCACGGAATCTTACCCATCTTCT TCACCAATATGGTTTGTGGACTCTGATGACCCAAATCTGACATCAGTTCTGGAACGCTTAGAAGATACTA AGAACAACAGTTCGCTCCGTCAGCAATTGAAGTGGTTGATATGTGACCTCTGCAGATTATATAACCTTCC TAAGCACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACTGAAGAAGTA ACTTCAGAAGAAGAGGAAGAGGAGGAGATGGCTGAAGATATAGAAGACTTGGATCACTATGAGATGAAGG AAGAAGAACCTATTAATGGGAAAAAGTCAGAGGATGAAGGGATTGAAAAAGAAAATTTGGCAATATTAGA GAAAATCAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCTGGGTCAGTACAAGCATCAGAC AGACTGATGAAGGAGCTCAGGGACGTCTACAGATCACAGAGCTACAAGGCAGGGATTTATTCAGTGGAGC TAACAAATGACAGCTTATATGACTGGCATGTCAAACTACACAAGGTTGACTCTGATAGTCCTTTGCACAG TGATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAAT TTTCCATTTGATCCTCCGTTTGTTAGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTGTTGGGTGGAG GAGCACTTTGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAATCTGTCATCAT GCAGATCAATGCCACCTTAGTGAAAGGCAAAGCCCGGGTGCAGTTTGGAGCAAATAAGAATCAGTATAAT CTAGCACGAGCCCAACAGTCCTATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACACCTC CAAAGGAAGATGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205892 representing NM_180600
Red=Cloning site Green=Tags(s) MSVSGLKAELKFLASIFDKNHERFRIVSWKLDELHCQFLVPPPPPPPGSSLSPPPPLTLHCNITESYPSS SPIWFVDSDDPNLTSVLERLEDTKNNSSLRQQLKWLICDLCRLYNLPKHLDVEMLDQPLPTGQNGTTEEV TSEEEEEEEMAEDIEDLDHYEMKEEEPINGKKSEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASD RLMKELRDVYRSQSYKAGIYSVELTNDSLYDWHVKLHKVDSDSPLHSDLQILKEKEGIEYILLNFSFKDN FPFDPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSAYSIESVIMQINATLVKGKARVQFGANKNQYN LARAQQSYNSIVQIHEKNGWYTPPKEDG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_180600 |
ORF Size | 1134 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_180600.1, NP_850931.1 |
RefSeq Size | 2933 bp |
RefSeq ORF | 1137 bp |
Locus ID | 109161 |
UniProt ID | Q8K2Z8 |
Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201436 | Ube2q2 (untagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 2 (Ube2q2), (10ug) |
CNY 3,656.00 |
|
MR205892 | Ube2q2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 2 (Ube2q2) |
CNY 3,656.00 |
|
MR205892L3 | Lenti ORF clone of Ube2q2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 2 (Ube2q2) |
CNY 5,230.00 |
|
MR205892L4 | Lenti ORF clone of Ube2q2 (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 2 (Ube2q2) |
CNY 5,230.00 |