Gfra3 (BC066202) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	GFRalpha3; Y15110
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206230 representing BC066202 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTCTCCTGGAGCCCGCGACCTCCACTGCTGATGATCCTGCTACTGGTGCTGTCGTTGTGGCTGC CACTTGGAGCAGGAAACTCCCTTGCCACAGAGAACAGGTTTGTGAACAGCTGTACCCAGGCCAGAAAGAA ATGCGAGGCTAATCCCGCTTGCAAGGCTGCCTACCAGCACCTGGGCTCCTGCACCTCCAGTTTAAGCAGG CCGCTGCCCTTAGAGGAGTCTGCCATGTCTGCAGACTGCCTAGAGGCAGCAGAACAACTCAGGAACAGCT CTCTGATAGACTGCAGGTGCCATCGGCGCATGAAGCACCAAGCTACCTGTCTGGACATTTATTGGACCGT TCACCCTGCCCGAAGCCTTGGTGACTACGAGTTGGATGTCTCACCCTATGAAGACACAGTGACCAGCAAA CCCTGGAAAATGAATCTTAGCAAGTTGAACATGCTCAAACCAGACTCGGACCTCTGCCTCAAATTTGCTA TGCTGTGTACTCTTCACGACAAGTGTGACCGCCTGCGCAAGGCCTACGGGGAGGCATGCTCAGGGATCCG CTGCCAGCGCCACCTCTGCCTAGCCCAGCTGCGCTCCTTCTTTGAGAAGGCAGCAGAGTCCCACGCTCAG GGTCTGCTGCTGTGTCCCTGTGCACCAGAAGATGCGGGCTGTGGGGAGAGGCGGCGTAACACCATCGCCC CCAGTTGCGCCCTGCCTTCTGTAACCCCCAATTGCCTGGATCTGCGGAGCTTCTGCCGTGCGGACCCTTT GTGCAGATCACGCCTGATGGACTTCCAGACCCACTGTCATCCTATGGACATCCTTGGGACTTGTGCAACT GAGCAGTCCAGATGTCTGCGGGCATACCTGGGGCTGATTGGGACTGCCATGACCCCAAACTTCATCAGCA AGGTCAACACTACTGTTGCCTTAAGCTGCTCCTGCCGAGGCAGCGGCAACCTACAGGACGAGTGTGAACA GCTGGAAAGGTCCTTCTCCCAGAACCCCTGCCTCGTGGAGGCCATTGCAGCTAAGATGCGTTTCCACAGA CAGCTCTTCTCCCAGGACTGGGCAGACTCTACTTTTTCAGTGGTGCAGCAGCAGAACAGCAACCCTGCTC TGAGACTGCAGCCCAGGCTACCCATTCTTTCTTTCTCCATCCTTCCCTTGATTCTGCTGCAGACCCTCTG G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206230 representing BC066202 Red=Cloning site Green=Tags(s) MGLSWSPRPPLLMILLLVLSLWLPLGAGNSLATENRFVNSCTQARKKCEANPACKAAYQHLGSCTSSLSR PLPLEESAMSADCLEAAEQLRNSSLIDCRCHRRMKHQATCLDIYWTVHPARSLGDYELDVSPYEDTVTSK PWKMNLSKLNMLKPDSDLCLKFAMLCTLHDKCDRLRKAYGEACSGIRCQRHLCLAQLRSFFEKAAESHAQ GLLLCPCAPEDAGCGERRRNTIAPSCALPSVTPNCLDLRSFCRADPLCRSRLMDFQTHCHPMDILGTCAT EQSRCLRAYLGLIGTAMTPNFISKVNTTVALSCSCRGSGNLQDECEQLERSFSQNPCLVEAIAAKMRFHR QLFSQDWADSTFSVVQQQNSNPALRLQPRLPILSFSILPLILLQTLW TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066202
ORF Size	1193 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066202, AAH66202
RefSeq Size	2006 bp
RefSeq ORF	1193 bp
Locus ID	14587
Gene Summary	This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]

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