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Isgf3g (BC005435) Mouse Tagged ORF Clone
CAT＃: MG206259
- TrueORF®
Isgf3g (tGFP-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete
ORF Plasmid: DDK
"BC005435" in other vectors (4)
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CNY 2,470.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p48, Irf-9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206259 representing BC005435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGGCAAAGTACGCTGCACCCGAAAGCTGCGGAGCTGGATCGTGGAGCAGGTGGAGAGTGGCC ATTTCCCAGGGGTGTGCTGGGACGATGCAGCCAAGACCATGTTCCGGATTCCCTGGAAGCATGCAGGCAA GCAAGACTTCCGAGAGGACCAGGATGCTGCCATATTCAAGGCTTGGGCACTGTTTAAGGAAAAGCACAAA GATGGGGACATAGGACACCCCGCTGTCTGGAAGACTCGCCTACGCTGTGCCCTCAACAAGAGTTCCGAAT TTGAGGAGGTTCCCGAGAGAGGTCGTATGGATGTTGCTGAACCCTACAAAGTATATCGAATACTGCCAGC AGGAACCCTCCCTAACCAACCACGGAACCAGAAATCACCATGCAAGCGAAGTATCAGTTGTGTGTCACCT GAGAGGGAAGAAAATATGGAAAATGGGAGGACCAATGGCGTTGTAAACCACTCAGACAGTGGCAGCAACA TAGGCGGTGGTGGCAATGGCAGCAACAGGAGCGACAGCAACAGCAACTGCAACTCTGAGCTAGAGGAGGG AGCTGGCACAACTGAGGCCACCATTAGAGAGGACCCAGTGTTCCTGGAGCATCAACTTCCTCTGAACTCA GACTACTCGCTGCTGCTCACCTTCATCTATGGTGGCCGAGTGGTGGGTAAGACCCAGGTGCACAGCCTAG ACTGTCGGCTCGTGGCTGAGCGCTCAGACTCGGAGAGCAGCATGGAGCAGGTGGAGTTTCCCAAACCCGA CCCACTGGAGCCTACCCAGCACCTGCTGAATCAGCTTGACAGAGGCGTCCTGGTGGCCAGCAATTCCAGA GGCCTCTTTGTTCAGCGCCTTTGCCCCATCCCCATCTCCTGGAATGCACCAGAGGCCCCACCCGGGCCTG GTCCTCATCTGCTGCCCAGCAATAAGTGTGTGGAGCTCTTCAAGACCACCTACTTCTGTAGAGATTTGGC CCAGTACTTCCAGGGCCAGGGGCCCCCACCCAAGTTCCAAGCAACCCTACATTTCTGGGAGGAGAGTCCT GGCTCTAGCCATAGCCAAGAGAATCTCATCACAGTGCAGATGGAGCAGGCCTTTGCCCGACATTTACTGG AGAAGATTCCAGAAGAGGAGAAAGCTGCCTTGTTCCTGTTACAGCACACAGAGCAGTCACCCTCTGCTCT GGGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206259 representing BC005435
Red=Cloning site Green=Tags(s) MASGKVRCTRKLRSWIVEQVESGHFPGVCWDDAAKTMFRIPWKHAGKQDFREDQDAAIFKAWALFKEKHK DGDIGHPAVWKTRLRCALNKSSEFEEVPERGRMDVAEPYKVYRILPAGTLPNQPRNQKSPCKRSISCVSP EREENMENGRTNGVVNHSDSGSNIGGGGNGSNRSDSNSNCNSELEEGAGTTEATIREDPVFLEHQLPLNS DYSLLLTFIYGGRVVGKTQVHSLDCRLVAERSDSESSMEQVEFPKPDPLEPTQHLLNQLDRGVLVASNSR GLFVQRLCPIPISWNAPEAPPGPGPHLLPSNKCVELFKTTYFCRDLAQYFQGQGPPPKFQATLHFWEESP GSSHSQENLITVQMEQAFARHLLEKIPEEEKAALFLLQHTEQSPSALGH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC005435 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005435.1 |
| RefSeq Size | 2341 bp |
| RefSeq ORF | 1199 bp |
| Locus ID | 16391 |
| Gene Summary | Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200578 | Isgf3g (untagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete, (10ug) |
CNY 3,656.00 |
|
| MR206259 | Isgf3g (Myc-DDK-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete |
CNY 3,656.00 |
|
| MR206259L3 | Lenti ORF clone of Isgf3g (Myc-DDK-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete |
CNY 4,180.00 |
|
| MR206259L4 | Lenti ORF clone of Isgf3g (mGFP-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete |
CNY 4,180.00 |
