Tead1 (BC060138) Mouse Tagged ORF Clone

CAT#: MG206462

  • TrueORF®

Tead1 (tGFP-tagged) - Mouse TEA domain family member 1 (cDNA clone MGC:73506 IMAGE:6410573)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms TEF-1, TEAD-1, mTEF-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206462 representing BC060138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGGATGAGCGACTCGGCAGATAAGCCGATTGACAACGACGCGGAGGGCGTCTGGAGTCCTGATA
TTGAGCAGAGTTTCCAGGAGGCCCTGGCTATCTATCCGCCGTGTGGGAGGAGAAAAATCATCTTATCAGA
CGAAGGCAAAATGTATGGTAGAAATGAATTGATAGCCAGATACATCAAACTCAGGACGGGAAAGACAAGG
ACCAGGAAGCAGGTGTCTAGTCACATTCAGGTTCTTGCCAGAAGGAAATCTCGTGATTTTCATTCCAAGC
TGAAGGATCAGACTGCCAAGGACAAGGCCCTGCAGCACATGGCTGCCATGTCATCAGCCCAGATCGTCTC
GGCTACTGCCATCCACAACAAGCTGGGGCTGCCTGGGATTCCACGCCCCACCTTCCCGGGGGGTCCGGGG
TTCTGGCCTGGGATGATACAGACAGGACAGCCAGGATCCTCACAAGACGTCAAGCCCTTTGTGCAGCAGG
CCTACCCCATCCAGCCAGCAGTCACAGCCCCCATTCCAGGGTTTGAGCCTACGTCAGCCCCAGCCCCCTC
AGTTCCTGCCTGGCAGGGCCGATCCATTGGCACAACCAAGCTTCGCCTGGTGGAATTCTCCGCTTTCCTT
GAACAGCAGAGAGACCCAGACTCGTACAACAAACACCTCTTCGTGCACATCGGGCATGCCAACCATTCTT
ACAGTGACCCGTTGCTCGAATCTGTGGACATTCGTCAGATATATGACAAATTTCCTGAAAAGAAAGGTGG
CTTGAAGGAGCTGTTTGGAAAGGGCCCTCAAAACGCCTTCTTCCTCGTCAAATTCTGGGCGGACTTAAAC
TGCAATATCCAAGACGACGCCGGGGCCTTTTATGGTGTGAGCAGTCAGTATGAGAGTTCTGAGAACATGA
CAGTTACCTGTTCCACCAAAGTGTGCTCCTTTGGGAAACAAGTAGTAGAAAAAGTAGAGACGGAGTATGC
GAGGTTCGAGAATGGTCGATTCGTGTACCGAATAAACCGCTCGCCAATGTGTGAATATATGATCAACTTC
ATCCACAAGCTCAAACACCTACCAGAGAAATATATGATGAACAGTGTTTTGGAAAACTTCACCATATTAT
TGGTGGTAACAAACAGGGATACACAAGAAACTCTGCTCTGCATGGCCTGTGTATTTGAAGTCTCGAATAG
CGAACACGGAGCACAGCACCATATCTACAGGCTTGTGAAGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206462 representing BC060138
Red=Cloning site Green=Tags(s)

MERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTR
TRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGGPG
FWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPTSAPAPSVPAWQGRSIGTTKLRLVEFSAFL
EQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLN
CNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINF
IHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060138
ORF Size 1235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060138, AAH60138
RefSeq Size 2091 bp
RefSeq ORF 1235 bp
Locus ID 21676
Gene Summary Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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