Cept1 (BC021753) Mouse Tagged ORF Clone
CAT#: MG206581
- TrueORF®
Cept1 (tGFP-tagged) - Mouse choline/ethanolaminephosphotransferase 1 (cDNA clone MGC:25615 IMAGE:4167222)
ORF Plasmid: DDK
"BC021753" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,660.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 9930118K05Rik; mCEPT1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206581 representing BC021753
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGGCATCGGTCAACAAGGAAACGATGTGGAGATTCTCACCCAGAGTCCCCTGTGGGCTTTGGGC ATATGAGTACTACAGGGTGTGTATTAAATAAATTGTTTCAGTTACCGACACCACCACTGTCAAGACACCA GTTAAAACGGCTAGAAGAACACAGGTATCAGAGTGCTGGACGGTCCCTTCTCGAGCCCTTAATGCAAGGA TACTGGGAATGGCTAGTTGGAAGAGTACCCTCATGGATTGCCCCAAATCTCATCACCATCATTGGACTAT CAATAAATATCTGTACAACTATTTTGTTAGTCTTCTACTGCCCTACAGCTACAGAGCAGGCACCTCTGTG GGCTTATATTGCCTGTGCTTGTGGCCTTTTCATTTACCAGTCTCTGGATGCCATAGATGGGAAACAGGCC AGAAGAACTAACAGTAGCTCTCCTCTGGGAGAACTTTTTGATCATGGCTGTGACTCACTATCGACAGTTT TTGTGGTTCTTGGAACTTGTATTGCAGTACAACTTGGGACAAATCCTGACTGGATGTTTTTTTGTTGTTT TGCTGGGACATTCATGTTCTATTGTGCACACTGGCAAACATATGTTTCTGGAACATTGCGATTTGGAATA TTCGATGTTACAGAGTCCCAGATCCTAATTATATTATTCCAGCTTCTCTCAGGAACCGTGGGGCCTTGGT TCTGGAACTTCACGATTCCTGTGTTGAATATTCAAATGAAACTTCTTCCTGCACTTTGTACTGTGGCAGG GACCATATTTTCCTGTACAAATTACTTCCGTGTAATCTTCACAGGTGGTGTTGGCAAAAATGGGTCAACC ATAGCAGGAACAAGTGTCCTTTCTCCTTTTCTGCATATTGGATCAGTGATCACGTTAGCTGTTATGATCT ACAAGAAATCAGCAGTTCAGCTTTTTGAAAAGCATCCATGTCTTTATATACTGACATTTGGTTTTGTGTC GGCTAAAATCACTAATAAGCTTGTGGTTGCTCACATGACGAAAAGTGAAATGCATTTGCATGACACAGCA TTCATAGGTCCAGCACTTCTGTTTCTGGACCAGTATTTTAACAGCTTCATTGATGAATATATAGTACTTT GGATTGCCTTGATCTTCTCTTTCTTTGATTTGATTCGTTACTGTGTCAGTGTTTGCAATCAGATTGCATC TCACCTGCATATACATGTCTTCAGAATTAAAGCCTCTACAGCTCACTCTAATCATCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206581 representing BC021753
Red=Cloning site Green=Tags(s) MSGHRSTRKRCGDSHPESPVGFGHMSTTGCVLNKLFQLPTPPLSRHQLKRLEEHRYQSAGRSLLEPLMQG YWEWLVGRVPSWIAPNLITIIGLSINICTTILLVFYCPTATEQAPLWAYIACACGLFIYQSLDAIDGKQA RRTNSSSPLGELFDHGCDSLSTVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTYVSGTLRFGI FDVTESQILIILFQLLSGTVGPWFWNFTIPVLNIQMKLLPALCTVAGTIFSCTNYFRVIFTGGVGKNGST IAGTSVLSPFLHIGSVITLAVMIYKKSAVQLFEKHPCLYILTFGFVSAKITNKLVVAHMTKSEMHLHDTA FIGPALLFLDQYFNSFIDEYIVLWIALIFSFFDLIRYCVSVCNQIASHLHIHVFRIKASTAHSNHH TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC021753 |
ORF Size | 1250 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC021753, AAH21753 |
RefSeq Size | 2038 bp |
RefSeq ORF | 1250 bp |
Locus ID | 99712 |
Gene Summary | Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR206581 | Cept1 (Myc-DDK-tagged) - Mouse choline/ethanolaminephosphotransferase 1 (cDNA clone MGC:25615 IMAGE:4167222) |
CNY 3,656.00 |
|
MR206581L3 | Lenti ORF clone of Cept1 (Myc-DDK-tagged) - Mouse choline/ethanolaminephosphotransferase 1 (cDNA clone MGC:25615 IMAGE:4167222) |
CNY 4,280.00 |
|
MR206581L4 | Lenti ORF clone of Cept1 (mGFP-tagged) - Mouse choline/ethanolaminephosphotransferase 1 (cDNA clone MGC:25615 IMAGE:4167222) |
CNY 4,280.00 |