Pgk1 (BC108372) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Pgk-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206593 representing BC108372 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG ACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACAACCAAAGGATCAAGGCTGCTGTTCCAAGCAT CAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGCCACCTGGGCCGGCCTGATGGTGTT CCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCTGAACTCAAATCTCTGCTGGGCAAGGATGTTC TGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT CCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGAAACAAGGTT AAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCACTGTCCAAACTAGGAGATGTCTATGTCAATG ATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT ATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA TGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT GTATGATGAAGAAGGAGCCAAGATTGTCAAAGATCTCATGTCCAAAGCTGAGAAAAATGGTGTGAAGATT ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCCAAGACTGGCCAAGCTACTGTGG CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGCAAGAAATATGCCGAGGC TGTGGGTCGAGCTAAGCAGATTGTTTGGAATGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG GGAACCAAGTCACTCATGGATGAGGTGGTGAAAGCCACTTCTAGGGGTTGCATCACTATCATAGGTGGTG GAGACACTGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG TGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206593 representing BC108372 Red=Cloning site Green=Tags(s) MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGV PMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKV KAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLA ILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKI TLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFAR GTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC108372
ORF Size	1251 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC108372, AAI08373
RefSeq Size	1765 bp
RefSeq ORF	1253 bp
Locus ID	18655
Gene Summary	The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]

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