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Ppp4r2 (BC110429) Mouse Tagged ORF Clone
CAT＃: MG206608
- TrueORF®
Ppp4r2 (tGFP-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:117881 IMAGE:5350332)
ORF Plasmid: DDK
"BC110429" in other vectors (4)
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CNY 4,560.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BE691708; C230060M08Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206608 representing BC110429
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTCGAGAGGCTTCAGGAGGCGCTGAAAGATTTTGAGAAGAGAGGGAAAAAGGAAGTTTGTCCTG TACTGGATCAGTTTCTCTGTCATGTAGCTAAAACTGGAGAAACAATGATTCAGTGGTCCCAATTTAAAGG CTATTTCATTTTCAAACTGGAGAAAGTGATGGATGATTTCAGAACTTCAGCTCCTGAACCAAGAGGTCCT CCCAATCCTAATGTTGAATATATTCCCTTTGATGAAATGAAGGAAAGAATACTGAAAATTGTCACTGGAT TTAATGGTATCCCTTTTACTATTCAGCGGCTATGTGAATTGCTAACGGATCCGAGAAGAAACTATACAGG AACAGACAAGTTTCTCAGAGGAGTAGAGAAGAATGTGATGGTTGTTAGCTGCGTTTGTCCATCCTCAGAG AAGAACAATTCTAATAGCTTAAATAGAATGAATGGTGTTATGTTTCCTGGAAACTCACCAAACTATACTG ACAGGTCTAATATAAACGGGCCTGGAACACCTAGGCCACTTAATCGACCAAAGCTTTCTTTGTCAGCCCC CTTGACAACAAATGGTTTGCCTGAGAGCACAGATAGCAAAGATTCTGAGCTGCAGCTAAGTGAAGAGAAA GGCCACAGTGATTCTTCAGCCTCTGAATCAGAAGTTTCCTTACTGAGCCCTGTTAAAAATAAACATCCAG ATGAAGATGCTGTGGAGTCTGAGGAACATGAGGTGAAAAGACTGAAGTTTGACAAAGAAGGTGACGTCAG AGAGACAGCTAGCCAAACGGTGTCTGGTGAAGTCTCTTCAGTTAGAGCAGAGGAAACGGAAACAGCAGCT CCCCCTCCTGACAAGGACAGAGAAAGTCGAACCAGGCAGCACTGTACAGAAGAGGAGGAGGAGGAGGAGG AAGAAGAGGAAGAGGAGGAGGAAGAATCGTTTATGACACCAAGAGAAATGGTCCCAGAAAGAAAAAATCA AGAAAAGGAATCTGATGACGCCTTAACTGTGAATGAAGAGACTTCAGAGGAGAGCCATCAGATGGAAGGC TCTGGTGTGTCTCCAGCTCAGACAGACTCCACTTCAGAAAGGAGTGACAGTGCAGGGGCCTCAAGGAGTG GCTCTGACTGCCTGGAGACACAGGAGTCAGGAGGGCCCCCTTCCAGTAAGACTGGAGAGAGTGTGTCAGT GCCGTCGTCCATGGAGAGTGAGGAAGCCACAGAAGTCACAGATGACCCAATGGAGCAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206608 representing BC110429
Red=Cloning site Green=Tags(s) MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGP PNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVCPSSE KNNSNSLNRMNGVMFPGNSPNYTDRSNINGPGTPRPLNRPKLSLSAPLTTNGLPESTDSKDSELQLSEEK GHSDSSASESEVSLLSPVKNKHPDEDAVESEEHEVKRLKFDKEGDVRETASQTVSGEVSSVRAEETETAA PPPDKDRESRTRQHCTEEEEEEEEEEEEEEEESFMTPREMVPERKNQEKESDDALTVNEETSEESHQMEG SGVSPAQTDSTSERSDSAGASRSGSDCLETQESGGPPSSKTGESVSVPSSMESEEATEVTDDPMEQD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC110429 |
| ORF Size | 1251 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC110429.1 |
| RefSeq Size | 2959 bp |
| RefSeq ORF | 1253 bp |
| Locus ID | 232314 |
| Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218614 | Ppp4r2 (untagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:117881 IMAGE:5350332), (10ug) |
CNY 6,940.00 |
|
| MR206608 | Ppp4r2 (Myc-DDK-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:117881 IMAGE:5350332) |
CNY 4,180.00 |
|
| MR206608L3 | Lenti ORF clone of Ppp4r2 (Myc-DDK-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:117881 IMAGE:5350332) |
CNY 6,080.00 |
|
| MR206608L4 | Lenti ORF clone of Ppp4r2 (mGFP-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:117881 IMAGE:5350332) |
CNY 6,080.00 |
