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Elmo1 (BC031782) Mouse Tagged ORF Clone
CAT＃: MG206637
- TrueORF®
Elmo1 (tGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C
ORF Plasmid: DDK
"BC031782" in other vectors (4)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CED-12, 6330578D22Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206637 representing BC031782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACTAAGATGGACCCCCAGGACCAGGCCCAAAGGGACATCATATTTGAACTTCGAAGAATTGCTT TCGATGCAGAGTCAGAACCTAATAACAGCAGTGGCAGCATGGAGAAACGCAAGTCCATGTACACTCGGGA TTATAAAAAACTTGGCTTCATTAATCACGTCAATCCTGCCATGGACTTCACACAGACCCCTCCTGGGATG TTGGCTCTGGACAACATGCTGTATTTCGCTAAGCACCACCAGGATGCATACATCCGGATCGTGCTGGAGA ACAGCAGCCGAGAAGATAAACATGAGTGCCCCTTCGGCCGCAGCAGTATAGAGCTGACCAAGATGCTGTG TGAGATCCTGAAAGTGGGCGAGCTGCCTAGTGAGACCTGCAACGACTTTCACCCGATGTTCTTCACCCAT GACAGATCTTTTGAGGAATTCTTCTGCATTTGCATTCAACTCCTGAACAAAACATGGAAGGAAATGAGGG CAACATCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGAGCGCTTACAACCAA GCCTAGTTCCCTGGACCAGTTCAAAAGCAAGCTGCAAAACCTGAGCTACACCGAGATCCTGAAAATCCGC CAGTCTGAGAGGATGAACCAGGAAGATTTCCAGTCTCGCCCGATTTTGGAACTAAAGGAGAAGATCCAGC CAGAAATCTTAGAGCTGATTAAACAGCAGCGCCTGAACCGCCTTGTGGAAGGGACCTGCTTTAGGAAACT CAATGCTCGCCGGAGACAAGACAAGTTTTGGTATTGTCGGCTTTCACCAAATCACAAGGTCTTACATTAT GGTGACTTGGAAGAGAGCCCCCAAGGAGAAGTGCCCCACGATTCCCTGCAGGACAAATTGCCGGTGGCAG ATATCAAAGCAGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAAACAA GGAGGTGCTTGAACTTGCTTTCTCCATCTTATACGACTCAAATTGCCAACTGAACTTCATTGCTCCTGAT AAGCATGAGTACTGCATCTGGACAGATGGGCTGAATGCACTGCTTGGGAAGGACATGATGAGTGACCTGA CACGCAATGACCTGGACACCCTGCTGAGCATGGAGATCAAGCTTCGCCTGCTGGACCTGGAAAACATCCA GATCCCGGATGCACCTCCGCCTATCCCCAAGGAACCTAGCAACTATGACTTTGTCTATGACTGTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206637 representing BC031782
Red=Cloning site Green=Tags(s) MMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKSMYTRDYKKLGFINHVNPAMDFTQTPPGM LALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIELTKMLCEILKVGELPSETCNDFHPMFFTH DRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMRALTTKPSSLDQFKSKLQNLSYTEILKIR QSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHY GDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGALKQNKEVLELAFSILYDSNCQLNFIAPD KHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLDLENIQIPDAPPPIPKEPSNYDFVYDCN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031782 |
| ORF Size | 1257 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031782, AAH31782 |
| RefSeq Size | 2540 bp |
| RefSeq ORF | 1259 bp |
| Locus ID | 140580 |
| Gene Summary | Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217958 | Elmo1 (untagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C, (10ug) |
CNY 6,940.00 |
|
| MR206637 | Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C |
CNY 3,656.00 |
|
| MR206637L3 | Lenti ORF clone of Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C |
CNY 6,056.00 |
|
| MR206637L4 | Lenti ORF clone of Elmo1 (mGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C |
CNY 6,056.00 |
