Tdg (BC085470) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	E130317C12Rik; JZA-3; Jza1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206698 representing BC085470 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCAGAGGCCGCGCGCAGCTATTCTCTGGAGCAAGTTCAAGCTTTGTATTCATTTCCATTTCAAC AAATGATGGCAGAAGTTCCTAACATGGCAGTCACGACTGGACAGCAGGTGCCAGCAGTAGCTCCTAACAT GGCAACCGTGACAGAACAGCAGGTGCCCGAAGACGCTCCTGTCCAGGAACCTGCACCAGAAGCTCCAAAG AGAAGGAAAAGGAAACCCAGAGCAGCAGAGCCCCAGGAACCAGTGGAGCCCAAAAAACCTGCTACGTCGA AGAAATCCGGCAAGTCTACAAAATCAAAGGAAAAGCAGGAGAAAATCACAGACGCGTTTAAAGTGAAAAG GAAAGTGGACCGCTTCAACGGCGTCTCTGAAGCTGAGCTTCTGACCAAGACTCTTCCTGACATTTTGACC TTCAATCTGGATATTGTGATCATTGGCATTAACCCGGGATTAATGGCTGCTTACAAAGGACATCACTACC CTGGGCCTGGAAATCACTTCTGGAAGTGTCTGTTCATGTCGGGGCTGAGTGAGGTGCAGCTGAATCACAT GGATGACCACACCTTACCCGGCAAGTACGGCATCGGATTCACCAACATGGTGGAACGGACGACGCCGGGC AGCAAGGATCTGTCTATTAAAGAGTTCCGGGAAGGAGGGCGCATCCTGGTGCAGAAACTGCAGAAATATC AGCCACGAATAGCGGTGTTTAATGGAAAATGTATTTATGAAATTTTCAGTAAAGAAGTTTTTGGAGTAAA GGTTAAGAACTTGGAATTTGGGCTTCAACCCCACAAGATCCCAGACACAGAAACTCTGTGCTACGTCATG CCGTCGTCCAGCGCCAGATGTGCTCAGTTTCCCCGGGCCCAGGACAAAGTTCATTACTACATTAAGCTGA AGGACTTGAGAGACCAACTGAAAGGCATTGAACGCAACGCGGACGTTCAGGAAGTGCAGTATACATTTGA CCTGCAGCTTGCGCAAGAGGACGCAAAGAAGATGGCTGTTAAGGAAGAAAAGTATGATCCAGGCTATGAG GCAGCTTACGGCGGTGCCTATGGGGAAAACCCATGTAATGGGGAACCTTGTGGCATTGCTTCAAATGGGC TAACAGCTCACAGTGCGGAGCCGAGAGGAGAAGCGGCCCCCAGCGATGTTCCGAATGGGCAGTGGATGGC ACAGTCGTTTGCAGAGCAGATCCCTTCTTTTAATAATTGTGGGACCCGAGAGCAGGAAGAAGAGAGCCAC GCT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206698 representing BC085470 Red=Cloning site Green=Tags(s) MDAEAARSYSLEQVQALYSFPFQQMMAEVPNMAVTTGQQVPAVAPNMATVTEQQVPEDAPVQEPAPEAPK RRKRKPRAAEPQEPVEPKKPATSKKSGKSTKSKEKQEKITDAFKVKRKVDRFNGVSEAELLTKTLPDILT FNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPG SKDLSIKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVM PSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNADVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYE AAYGGAYGENPCNGEPCGIASNGLTAHSAEPRGEAAPSDVPNGQWMAQSFAEQIPSFNNCGTREQEEESH A SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC085470
ORF Size	1265 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC085470, AAH85470
RefSeq Size	2967 bp
RefSeq ORF	1265 bp
Locus ID	21665
Gene Summary	DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine (PubMed:21817016). Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC (PubMed:21722948). Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.[UniProtKB/Swiss-Prot Function]

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