Mapk9 (BC028341) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	JNK2, p54aSAPK
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206734 representing BC028341 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAGTAAAAGCGATGGTCAGTTTTACAGTGTGCAGGTGGCGGACTCAACTTTCACTGTTCTAA AACGTTACCAGCAACTGAAACCGATCGGCTCTGGAGCCCAAGGAATTGTTTGTGCTGCTTTTGATACAGT TCTTGGGATAAATGTGGCTGTCAAGAAGTTAAGTCGTCCTTTTCAGAACCAAACGCACGCAAAGAGAGCC TACCGTGAACTCGTCCTCTTAAAGTGTGTCAATCATAAAAACATAATTAGCTTGTTAAATGTGTTCACAC CACAAAAAACGCTAGAAGAATTTCAAGATGTGTACTTGGTAATGGAACTAATGGATGCTAACTTATGTCA GGTTATTCATATGGAACTGGACCATGAGAGAATGTCCTACCTTCTCTACCAGATGCTCTGTGGCATCAAG CATCTGCATTCAGCTGGTATCATTCATAGAGATTTGAAGCCTAGCAACATTGTAGTAAAATCAGACTGTA CCCTCAAGATCCTTGACTTTGGCCTGGCGCGAACAGCCTGTACCAACTTTATGATGACTCCCTATGTGGT AACTCGATACTATCGGGCTCCAGAAGTCATCCTGGGCATGGGCTACAAAGAGAATGTTGATATCTGGTCA GTGGGTTGCATCATGGGAGAGCTGGTGAAAGGTTGTGTGATATTCCAAGGTACTGACCATATTGATCAAT GGAATAAAGTTATTGAACAGCTAGGAACACCATCCGCAGAGTTCATGAAGAAACTTCAGCCAACTGTAAG GAATTATGTGGAAAACAGACCAAAGTACCCTGGAATCAAGTTTGAAGAGCTCTTTCCAGATTGGATATTT CCGTCAGAATCTGAGAGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCAAAAATGCTAGTGA TTGATCCAGACAAGCGGATCTCTGTGGACGAAGCCTTGCGCCACCCGTATATTACTGTTTGGTATGACCC CGCTGAAGCAGAAGCGCCACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAGCATGCGATTGAA GAGTGGAAAGAGCTAATTTACAAAGAAGTGATGGACTGGGAAGAAAGAAGCAAGAATGGGGTAAAAGACC AGCCTTCAGATGCAGCAGTAAGTAGCAAGGCTACTCCTTCTCAGTCGTCATCCATCAATGACATCTCATC CATGTCCACTGAGCACACCCTGGCCTCAGACACAGACAGCAGTCTCGATGCCTCAACAGGACCCCTGGAA GGCTGCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206734 representing BC028341 Red=Cloning site Green=Tags(s) MSDSKSDGQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRA YRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWS VGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIF PSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIE EWKELIYKEVMDWEERSKNGVKDQPSDAAVSSKATPSQSSSINDISSMSTEHTLASDTDSSLDASTGPLE GCR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC028341
ORF Size	1271 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028341, AAH28341
RefSeq Size	4372 bp
RefSeq ORF	1271 bp
Locus ID	26420
Gene Summary	Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (PubMed:29153991).[UniProtKB/Swiss-Prot Function]

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