Skp2 (BC092236) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4930500A04Rik; FBXL1; FWD1; p45
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206755 representing BC092236 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATAGGAAGCACCTTCAGGAGATTCCGGACCAGAGTGGCAACGTCACCACCAGCTTCACGTGGGGAT GGGATTCCAGCAAGACTTCTGAACTGCTATCAGGCATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGA CAGTGAGAACATCCCACATGGACTGCTCTCAAACCTCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTC AAGGGCAAAGGGAGTGACAAAGACTTTGTGATCATCCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAG GTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTCCTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCT GCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACCGCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTC GACCTCGCGGGTAAAAATCTGCACCCAGACGTGACTGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCC GCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGTGAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGA CCTGTCGAACTCAGTGATAAATGTGTCGAACCTCCATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAAT CTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCATTGTCAAGACTCTTGCACAGAATGAAAACTTGGTGC GACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAATCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAG ACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACTTCACTGAAAAGCACGTGCAAGCGGCTGTGGCACAT TTACCAAACACCATCACCCAGCTGAACCTCAGCGGCTACCGAAAGAACCTCCAGAAAACAGATCTTTGTA CCATAATTAAACGATGCCCCAACCTCATCCGCCTCGACTTAAGTGACAGTATCATGCTAAAGAATGACTG CTTTCCAGAATTTTTTCAACTCAACTACCTCCAACACCTCTCGCTCAGCCGGTGCTATGATATAATACCT GATACTCTACTTGAACTTGGAGAAATTCCTACACTAAAAACGCTACAAGTTTTTGGAATCGTGCCAGAGG GAACCCTTCAGCTACTGAGGGAAGCTCTTCCTCGGCTGCAGATTAACTGCGCCTATTTCACCACCATTGC AAGGCCAACTATGGACAGCAAGAAGAACCTGGAGATTTGGGGTATCAAGTGCCGACTGACTCTGCAAAAG CCCAGTTGTCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206755 representing BC092236 Red=Cloning site Green=Tags(s) MHRKHLQEIPDQSGNVTTSFTWGWDSSKTSELLSGMGVSALEKEEVDSENIPHGLLSNLGHPQSPPRKRV KGKGSDKDFVIIRRPKLSRENFPGVSWDSLPDELLLGIFSCLCLPELLRVSGVCKRWYRLSLDESLWQSL DLAGKNLHPDVTVRLLSRGVVAFRCPRSFMEQPLGESFSSFRVQHMDLSNSVINVSNLHKILSECSKLQN LSLEGLQLSDPIVKTLAQNENLVRLNLCGCSGFSESAVATLLSSCSRLDELNLSWCFDFTEKHVQAAVAH LPNTITQLNLSGYRKNLQKTDLCTIIKRCPNLIRLDLSDSIMLKNDCFPEFFQLNYLQHLSLSRCYDIIP DTLLELGEIPTLKTLQVFGIVPEGTLQLLREALPRLQINCAYFTTIARPTMDSKKNLEIWGIKCRLTLQK PSCL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC092236
ORF Size	1274 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC092236, AAH92236
RefSeq Size	3096 bp
RefSeq ORF	1274 bp
Locus ID	27401
Gene Summary	Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function]

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