Suclg2 (BC080781) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AF171077; AW556404; D6Wsu120e; SCS-betaG
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206910 representing BC080781 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCCCGGTGGCCATCGCGGCGCAGGCTGGGAAGCTTCTGCGAGAGCGAGCGCTGCGGCCGCTCC TGGCGGTCAGGTCCCAGGCCGGTCACTTAACCCCCCGAAGATGGCTGAACCTGCAGGAGTACCAGAGCAA GAAGCTCATGTCAGAGCACGGAGTGAGAGTGCAGAGGTTCTTTGTTGCCAACACTGCTAAAGAGGCTCTG GAGGCAGCCAAGAGATTGAATGCAAAAGAAATTGTTTTAAAAGCCCAGATCTTAGCTGGAGGAAGAGGAA AAGGTGTCTTCAATAGCGGTTTGAAAGGAGGTGTTCATTTAACAAAAGACCCTAAAGTAGTGGGAGAGTT GGCTCAACAGATGATTGGTTATAATCTAGCAACGAAACAAACTCCAAAAGAAGGTGTGAAAGTTAACAAG GTGATGGTTGCTGAAGCCCTGGATATTTCTAGAGAAACATACTTGGCCATTCTAATGGACCGGTCCCATA ATGGCCCTGTGATAGTGGGCAGTCCTCAGGGGGGCGTTGATATTGAGGAAGTGGCAGCTTCCAGTCCAGA ACTCATCTTTAAGGAGCAGATTGACATCTTTGAAGGGATAAAGGATAGCCAGGCCCAGCGCATGGCAGAA AATCCGGGCTTCCTTGGGTCTCTGAAAAACCAGGCTGCAGATCAGATTACAAAGCTGTACCATCTCTTCC TGAAGATTGATGCCACTCAGGTGGAAGTGAACCCCTTCGGTGAAACTCCAGAAGGACAAGTTGTCTGTTT TGATGCCAAGATAAACTTTGATGACAATGCTGAGTTCCGACAAAAGGACATATTTGCTATGGACGACAAA TCCGAGAATGAGCCCATTGAAAATGAAGCTGCCAGATACGATCTCAAGTATATAGGACTGGACGGGAACA TTGCCTGCTTCGTGAATGGTGCTGGGCTGGCCATGGCTACCTGCGACATCATCTTCCTGAACGGAGGGAA GCCAGCGAACTTCTTGGACCTTGGAGGTGGTGTAAAGGAAGCCCAAGTCTATGAAGCATTCAAACTGCTC ACGTCTGACCCCAAGGTGGAAGCCATTCTCGTCAATATCTTTGGTGGGATCGTCAACTGTGCCATCATTG CCAACGGAATCACCAAAGCCTGCCGGGAGCTGGAGCTCAAGGTGCCACTGGTAGTCCGGCTTGAAGGAAC TAATGTGCAGGAGGCTCAGAATATCCTCAAGAGCAGCGGACTCCCCATCACGTCAGCTGTGGACCTGGAG GATGCAGCCAAGAAAGCTGTTGCCAGTGTGGCAAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206910 representing BC080781 Red=Cloning site Green=Tags(s) MASPVAIAAQAGKLLRERALRPLLAVRSQAGHLTPRRWLNLQEYQSKKLMSEHGVRVQRFFVANTAKEAL EAAKRLNAKEIVLKAQILAGGRGKGVFNSGLKGGVHLTKDPKVVGELAQQMIGYNLATKQTPKEGVKVNK VMVAEALDISRETYLAILMDRSHNGPVIVGSPQGGVDIEEVAASSPELIFKEQIDIFEGIKDSQAQRMAE NPGFLGSLKNQAADQITKLYHLFLKIDATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQKDIFAMDDK SENEPIENEAARYDLKYIGLDGNIACFVNGAGLAMATCDIIFLNGGKPANFLDLGGGVKEAQVYEAFKLL TSDPKVEAILVNIFGGIVNCAIIANGITKACRELELKVPLVVRLEGTNVQEAQNILKSSGLPITSAVDLE DAAKKAVASVAKK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080781
ORF Size	1299 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080781, AAH80781
RefSeq Size	2243 bp
RefSeq ORF	1301 bp
Locus ID	20917
Gene Summary	GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function]

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