Kcnj14 (NM_145963) Mouse Tagged ORF Clone
CAT#: MG206914
- TrueORF®
Kcnj14 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14)
ORF Plasmid: DDK
"NM_145963" in other vectors (4)
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CNY 3,710.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A930026G01Rik; IRK4; Kir2.4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206914 representing NM_145963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGCTAGGGCCCTGCGCCGCCTGAGCGGCGCGCTGGAGCCAGGAAACAGTCGAGCTGGGGATG AGGAAGAGGCGGGGGCCGGACTGTGCCGCAATGGGTGGGCTCCTGGACCGGTAGCTGGGAGCCGGCGCCG CGGGCGCTTCGTCAAAAAAGATGGGCACTGCAATGTGCGCTTCGTGAATCTGGGCGGCCAGGGCGCGCGC TACCTAAGTGACCTGTTCACCACATGTGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCTCCTGTT CCTTCCTCGCCTCCTGGTTGCTCTTCGGCCTGACCTTCTGGCTCATCGCCTCGCTGCACGGCGACCTAGC TGCCCCACCGCCACCGGCTCCCTGTTTCTCGCAAGTGGCCAGCTTCCTGGCTGCCTTCCTCTTTGCACTG GAGACACAGACGTCCATAGGCTATGGTGTGCGCAGCGTCACCGAGGAGTGTCCAGCTGCTGTGGCCGCTG TGGTGCTGCAGTGCATCGCAGGCTGTGTGCTCGACGCCTTCGTCGTGGGTGCAGTTATGGCCAAGATGGC CAAGCCCAAGAAACGCAACGAGACACTAGTCTTCAGTGAGAACGCAGTGGTGGCTCTGCGAGACCACCGT CTCTGCCTCATGTGGCGGGTGGGCAACCTGCGACGCAGTCACCTGGTGGAAGCTCACGTGCGGGCCCAGC TGCTGCAGCCCCGTGTGACCCCCGAGGGTGAGTACATCCCACTGGACCACCAGGATGTAGATGTTGGTTT TGATGGAGGCACTGATCGCATCTTCCTCGTCTCCCCCATTACCATTGTGCATGAGATTGACTCTGCCAGT CCACTGTATGAGCTGGGGCGGGCCGAGCTGGCCCGGGCTGACTTTGAGCTGGTGGTCATTCTTGAAGGTA TGGTTGAGGCCACAGCCATGACTACACAGTGCCGCTCATCTTACCTCCCAGGTGAGCTGCTCTGGGGACA TCGCTTCGAGCCGGTCCTCTTCCAGCGTGGCTCCCAGTATGAGGTTGACTATCGCCACTTCCATCGAACA TATGAGGTCCCAGGGACACCAGTCTGCAGTGCCAAGGAGCTGGATGAACGGGCAGAGCAGGCTTCCCACA GCCCTAAGTCAAGTTTCCCTGGCTCCCTCACTGCATTTTGTTATGAGAATGAACTTGCTCTGAGCTGCTG CCAGGAGGAAGATGAGGAGGAGGACACTAAGGAGGGGACTTCAGCAGAGACCCCAGAGAGGGCTGCCAGC CCCCAAGCTCTTACACCAACCCTGGCTCTGACCCTGCCTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206914 representing NM_145963
Red=Cloning site Green=Tags(s) MGLARALRRLSGALEPGNSRAGDEEEAGAGLCRNGWAPGPVAGSRRRGRFVKKDGHCNVRFVNLGGQGAR YLSDLFTTCVDVRWRWMCLLFSCSFLASWLLFGLTFWLIASLHGDLAAPPPPAPCFSQVASFLAAFLFAL ETQTSIGYGVRSVTEECPAAVAAVVLQCIAGCVLDAFVVGAVMAKMAKPKKRNETLVFSENAVVALRDHR LCLMWRVGNLRRSHLVEAHVRAQLLQPRVTPEGEYIPLDHQDVDVGFDGGTDRIFLVSPITIVHEIDSAS PLYELGRAELARADFELVVILEGMVEATAMTTQCRSSYLPGELLWGHRFEPVLFQRGSQYEVDYRHFHRT YEVPGTPVCSAKELDERAEQASHSPKSSFPGSLTAFCYENELALSCCQEEDEEEDTKEGTSAETPERAAS PQALTPTLALTLPP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145963 |
ORF Size | 1302 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145963.2, NP_666075.1 |
RefSeq Size | 2639 bp |
RefSeq ORF | 1305 bp |
Locus ID | 211480 |
UniProt ID | Q8JZN3 |
Gene Summary | Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ14 gives rise to low-conductance channels with a low affinity to the channel blockers Barium and Cesium (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Lipin-1 expression is critical for keratinocyte differentiation
,Chae, M;Jung, JY;Bae, IH;Kim, HJ;Lee, TR;Shin, DW;,
J. Lipid Res.
,PubMed ID 26658689
[KCNJ14]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205408 | Kcnj14 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14), (10ug) |
CNY 3,656.00 |
|
MR206914 | Kcnj14 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14) |
CNY 3,656.00 |
|
MR206914L3 | Lenti ORF clone of Kcnj14 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14) |
CNY 5,230.00 |
|
MR206914L4 | Lenti ORF clone of Kcnj14 (mGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14) |
CNY 5,230.00 |