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Alg1 (BC011281) Mouse Tagged ORF Clone
CAT＃: MG206977
- TrueORF®
Alg1 (tGFP-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946
ORF Plasmid: DDK
"BC011281" in other vectors (4)
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CNY 4,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HMT1, HMAT1, MGC18946 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206977 representing BC011281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGTCCTGCGTGGCGCTGCTGGTCCTGGCGCTGCTGTTGCTGGTGCTGCTGCTGGGCTTGTGGA AGCGTGGGCGGCAAACGGGCAGGGCCCGGCATATGGTCGTCGTGGTGCTGGGCGACGTGGGCCGCAGCCC CCGCATGCAGTACCACGCGCTGTCATTGGCCCAGAGCGGCTTTTCGGTGACGCTGCTGGGGTTCTACACT GGGCCCCGAATTCTCCAGTATGGAGTCAAGGTTGTATTTCAGGCAGTGTACTTGCTGTGGAAGATGATGC GCATGGACCCTGCAGCCTACATCTTCCTCCAGAACCCTCCAGGTCTGCCTGCTATTGCCGTCTGCTGGTT TGTGGGCTGCATTTGTGGGAGCAAATTGGTCATCGACTGGCACAACTATGGCTACTCCATCATGGGCTTG GTCCATGGCCCCCGCCACCCCATTGTGCTGCTGGCCAAATGGTATGAGAAGTTCTTCGGGCGCCTGTCCC ACCTGAACCTGTGTGTGACCAATGCTATGCGGGAGGACCTGGCAGAGAACTGGTGTGTCAGGGCTGTGAC GCTCTACGACAAGCCAGCATCTTTCTTTAAGGAGACACCCCTGGACCTGCAGCATGAACTCTTTATGAAG CTGAGCCACACGTACTCTCCTTTCCAGAGCTGCTCAGATCCCTCACATCCTGACACAGAGAGGTCGGCCT TCACTGAGAGGGATTGTCAGAGCGGGGTTGTGAGGCGTCTGCATGGGCGGCCAGCACTGCTCGTGAGCAG CACAAGCTGGACAGAGGACGAGGACTTCTCCATCCTGCTTCGAGCATTAGAAAAGTTTGAGCAACAAGCA CTCACTGGAGACAGCCTCCCTTCCCTCGTCTGTGTGATAACAGGGAAGGGACCGCTCAGAGAGCACTACC GCCACCTCATCAGCCAGAAGCACCTTAGGCACATCCGGTTCTGCACCCCATGGCTGGAAGCCGAGGACTA CCCACTGCTTCTAGGGTCAGCGGATCTGGGTGTCTGTCTGCACATGTCCTCCAGTGGTCTGGACCTGCCC ATGAAGGTGGTGGACATGTTTGGGTGCCACTTGCCTGTGTGTGCCGTGAACTTCAAGTGTCTGCACGAGC TTGTAAGACATGGAGAGAATGGTCTGGTCTTCAAGGATGCTGAGGAGCTGGCAGCTCAGCTGCAGATGCT TTTCTCAAAGTTCCCAGACCCTGCTGGAAAGCTAAGCCAGTTCCGGAAGAAACTGCAGGAGTCGAGGCAG CAACGCTGGGATGAGAGCTGGCAGCACACTGTGCTCCCTCTGCTCACTCACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206977 representing BC011281
Red=Cloning site Green=Tags(s) MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYT GPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAVCWFVGCICGSKLVIDWHNYGYSIMGL VHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVRAVTLYDKPASFFKETPLDLQHELFMK LSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALLVSSTSWTEDEDFSILLRALEKFEQQA LTGDSLPSLVCVITGKGPLREHYRHLISQKHLRHIRFCTPWLEAEDYPLLLGSADLGVCLHMSSSGLDLP MKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQLQMLFSKFPDPAGKLSQFRKKLQESRQ QRWDESWQHTVLPLLTHR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011281 |
| ORF Size | 1314 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011281 |
| RefSeq Size | 1677 bp |
| RefSeq ORF | 1316 bp |
| Locus ID | 208211 |
| Gene Summary | Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218551 | Alg1 (untagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946, (10ug) |
CNY 6,940.00 |
|
| MR206977 | Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946 |
CNY 4,370.00 |
|
| MR206977L3 | Lenti ORF clone of Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946 |
CNY 6,270.00 |
|
| MR206977L4 | Lenti ORF clone of Alg1 (mGFP-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946 |
CNY 6,270.00 |
