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Ehd2 (BC027084) Mouse Tagged ORF Clone
CAT＃: MG207060
- TrueORF®
Ehd2 (tGFP-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224)
ORF Plasmid: DDK
"BC027084" in other vectors (4)
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CNY 4,940.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC25606, MGC38650 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207060 representing BC027084
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGGCGAGACAGAGGGCACCGTGCCCGGAAATGCTCTCGTTGTGGACCCAGAGAAGCCCTTCCGCA AACTCAACCCTTTTGGAAACACTTTTCTCAACAGGTTTATGTGCGCCCAACTCCCCAACCAGGTCCTGGA GAGCATCAGCATCATCGACACGCCTGGCATCCTATCAGGTGCAAAACAGAGAGTGAGCCGTGGCTACGAC TTCCCAGCTGTCCTGCGCTGGTTTGCTGAACGCGTGGACCTCATCATCTTGCTCTTTGATGCCCACAAGC TGGAGATTTCCGATGAATTCTCGGAGGCCATCGGTGCCTTGCGTGGCCACGAGGATAAGATCCGCGTGGT ACTCAACAAGGCAGACATGGTGGAGACACAGCAGCTGATGCGCGTCTACGGGGCGCTCATGTGGGCGCTG GGCAAGGTGGTGGGCACGCCTGAGGTCCTTCGCGTCTACATTGGCTCCTTCTGGTCCCAGCCGCTCCTTG TGCCGGACAACCGGCGTCTTTTCGAGCTGGAGGAACAGGACCTCTTCCGTGATATCCAAGGCCTACCGCG CCATGCGGCGCTGCGCAAGCTCAACGACCTAGTGAAACGGGCGCGGCTGGTGCGGGTACACGCTTACATC ATCAGCTACCTGAAGAAAGAGATGCCCACGGTGTTCGGGAAGGAAAACAAGAAGAAGCAGCTGATCCTGA AGCTGCCTGTCATCTTTGCGAAGATCCAGCTGGAGCACCACATCTCCCCTGGAGACTTTCCTGACTGCCA GAAGATGCAGGAGCTGTTGATGGCACATGACTTCACCAAGTTCCACTCGCTAAAGCCGAAGCTGCTGGAG GCGCTAGATGACATGCTGGCCCAAGACATTGCCAAGCTTATGCCGCTGCTTCGGCAGGAGGAGCTGGAGA GTGTCGAGGCTGGAGTGCAGGGCGGTGCGTTTGAGGGCACCCGCATGGGCCCCTTCGTGGAGCGGGGCCC TGATGAAGCAATAGAGGATGGTGAGGAGGGCTCAGAAGATGATGCCGAATGGGTGGTGACCAAGGACAAG TCCAAATACGATGAAATCTTCTACAACTTAGCTCCTGCTGATGGCAAGCTGAGTGGCTCTAAGGCCAAAA CCTGGATGGTGGGCACAAAGCTACCCAACTCTGTGCTGGGGAGAATCTGGAAGCTGAGCGATGTCGACCG AGACGGCATGCTGGATGATGAGGAGTTTGCCTTGGCCAGCCACCTCATTGAGGCTAAGCTCGAAGGCCAT GGTCTACCCACCAACCTACCCCGCCGCCTGGTGCCGCCTTCTAAGCGACGACAGAAGGGCTCTGCTGAA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207060 representing BC027084
Red=Cloning site Green=Tags(s) MHGETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSGAKQRVSRGYD FPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVLNKADMVETQQLMRVYGALMWAL GKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYI ISYLKKEMPTVFGKENKKKQLILKLPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLE ALDDMLAQDIAKLMPLLRQEELESVEAGVQGGAFEGTRMGPFVERGPDEAIEDGEEGSEDDAEWVVTKDK SKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALASHLIEAKLEGH GLPTNLPRRLVPPSKRRQKGSAE SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027084 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027084.1 |
| RefSeq Size | 2717 bp |
| RefSeq ORF | 1331 bp |
| Locus ID | 259300 |
| Gene Summary | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (PubMed:24508342). Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4 (PubMed:14676205). Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (PubMed:18502764, PubMed:21177873). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218657 | Ehd2 (untagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224), (10ug) |
CNY 6,940.00 |
|
| MR207060 | Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224) |
CNY 4,470.00 |
|
| MR207060L3 | Lenti ORF clone of Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224) |
CNY 6,370.00 |
|
| MR207060L4 | Lenti ORF clone of Ehd2 (mGFP-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224) |
CNY 6,370.00 |
