Ppp2r2b (BC026686) Mouse Tagged ORF Clone
CAT#: MG207118
- TrueORF®
Ppp2r2b (tGFP-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform (cDNA clone MGC:37708
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CNY 3,710.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SCA12, PR55-BETA, PP2A-PR55B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207118 representing BC026686
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATGCTTCTCTCGTTACCTGCCTTATATCTTCAGACCTCCAAACACCATCCTCTCTTCCAGCTGCC ATACTGAAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAGTTACTAGCGACGGGGGACAA GGGGGGTCGGGTCGTAATATTTCAGCGAGAACAGGAGAGTAAAAACCAGGTTCATCGTAGGGGTGAATAC AATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAAAGTTTAGAAATAGAAGAAA AAATCAATAAGATAAGATGGCTCCCTCAACAAAACGCAGCTTACTTTCTTCTGTCTACCAACGATAAAAC TGTGAAGCTGTGGAAAGTCAGTGAGCGGGATAAGAGGCCAGAAGGCTACAACCTGAAAGATGAGGAGGGC CGGCTCCGGGATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGG AGGCCACCCCACGAAGAGTGTTTGCCAATGCGCACACGTACCACATCAACTCTATATCTGTCAACAGCGA CTATGAGACCTACATGTCTGCTGATGACCTGAGGATTAACCTGTGGAACTTTGAGATAACCAATCAGAGT TTTAATATCGTGGACATCAAGCCAGCCAACATGGAGGAGCTCACAGAGGTGATCACTGCAGCTGAGTTCC ACCCCCACCACTGCAACACCTTCGTATACAGCAGCAGCAAGGGGACAATCCGGCTGTGTGACATGCGTGC ATCTGCGCTGTGTGACAGGCACACCAAGTTTTTTGAAGAACCGGAAGATCCAAGCAACAGATCGTTTTTC TCTGAAATCATCTCTTCAATTTCGGATGTGAAATTCAGCCACAGTGGGAGGTATATCATGACCAGAGACT ATCTGACCGTGAAAGTCTGGGACCTCAACATGGAAAATCGCCCCATTGAGACATACCAGGTTCATGACTA CCTCCGCAGCAAGTTGTGCTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAAT GGGTCAGACAGCGTCATCATGACGGGCTCCTACAATAACTTCTTCAGGATGTTCGACCGAAACACCAAGC GTGATGTGACCCTTGAGGCCTCAAGGGAAAACAGCAAACCCCGGGCTATCCTCAAACCCCGAAAAGTGTG CGTCGGGGGCAAGCGAAGAAAAGATGAGATCAGTGTTGACAGTCTGGACTTTAGCAAAAAGATCTTGCAT ACAGCTTGGCATCCTTCAGAAAACATTATAGCAGTGGCGGCTACGAATAACTTATATATATTCCAAGACA AGGTTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207118 representing BC026686
Red=Cloning site Green=Tags(s) MKCFSRYLPYIFRPPNTILSSSCHTEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEY NVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEG RLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQS FNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFF SEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWN GSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILH TAWHPSENIIAVAATNNLYIFQDKVN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC026686 |
ORF Size | 1340 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC026686, AAH26686 |
RefSeq Size | 2003 bp |
RefSeq ORF | 1340 bp |
Locus ID | 72930 |
Gene Summary | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.[UniProtKB/Swiss-Prot Function] |
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