Ttyh1 (BC046310) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4930459B04Rik; 6330408P11Rik; mTTY1; tty; TTY1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207183 representing BC046310 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCACCCCCGGGCTACCGACCCTCTGCCTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGACT TCCAGCTCCGCCCGGTGCCCAGCGGCTTTGCTCCCCGCGACCAAGAGTACCAGCAGGCCCTGCTGCTGGT GGCAGCCTTGGCTGGGCTTGGTTTAGGCCTGAGCCTCATCTTCATCGCTGTCTACCTCATCCGCTTCTGC TGCTGCAGGCCTCCAGAACCCCATGGGGCCAAGAGTCCCCCACCCGGAGGAGGTTGCGTCACCTGGAGCT GCATTGCTGCCCTTCTCGTGGGCTGTGCCGGCATTGGCATTGGTTTCTATGGTAACAGCGAGACCAGCGA TGGGGTGTCCCAGCTCAGCTCAGCACTGCTGCACGCCAACCACACGCTCAGCACCATCGATGACGTGGTG CTGGAAACGGTGGAGAGGCTGGGTGAGGCAGTGAAGACGGAGCTGACCACCCTGGAGGAAGTGCTGTCAG TCCGCATGGAGCTGGTGGCTGCCACCAGGGGAGCCCGAAGGCAGGCTGAAGCTGCTGCTCAGTACCTGCA AGGACTGGCCTTCTGGCAAGGAGTGTCCCTGAGCCCCGTACAGGTGGCTGAAGATGTGACTTTCGTGGAG GAGTACAGGTGGCTGGCCTATGTCCTCCTGCTGCTCTTGGTGCTTCTGGTTTGTCTCTTCACCCTCCTGG GCCTGGCAAAGCAGAGCAAATGGCTGGTGGTTGTGATGACTGCCATGAGTCTCCTGGTGCTTGTCCTGAG CTGGGGTTCTATGGGCCTAGAGGCTGCCACAGCTGTGGGCCTCAGTGACTTCTGCTCCAACCCAGACACC TATGTGCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTCAGCTATTATTTCCTGTGCA ACCAGGCGGTCTCCAACCCCTTCCAACAGAGGCTGACGCTGTCCCAGCGTGCTCTAGCCAGCATCCACTC TCAGCTGCAGGGCCTGGAGCGGGAAGCTATCCCTCAATTTTCTGCTGCACAGAAGCCCTTGTTATCCTTG GAGGAGACACTGAATGTGACAGAAAGAAGCTTCCACCAGTTGGTGGCGCTGCTGCACTGCCGCAGTCTGC ACAAGGACTATGGCTCAGCACTCCGAGGCCTGTGTGAAGATGCCCTAGAGGGCCTACTGTTCCTGATGCT CTTTTCTCTCCTGTCTGCGGGGGCCCTAGCCACCACTCTGTGCAGCCTGCCCCGCGCCTGGGCCCTCTTC CCACCCAGTGATGACTACGATGACACAGATGACGATGACCCTTTCAACCCTCAGGAATCCAAACGCTTTG TGCAGTGGCAGTCTTCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207183 representing BC046310 Red=Cloning site Green=Tags(s) MGAPPGYRPSAWVHLLHQLPRADFQLRPVPSGFAPRDQEYQQALLLVAALAGLGLGLSLIFIAVYLIRFC CCRPPEPHGAKSPPPGGGCVTWSCIAALLVGCAGIGIGFYGNSETSDGVSQLSSALLHANHTLSTIDDVV LETVERLGEAVKTELTTLEEVLSVRMELVAATRGARRQAEAAAQYLQGLAFWQGVSLSPVQVAEDVTFVE EYRWLAYVLLLLLVLLVCLFTLLGLAKQSKWLVVVMTAMSLLVLVLSWGSMGLEAATAVGLSDFCSNPDT YVLNLTQEETGLSSDILSYYFLCNQAVSNPFQQRLTLSQRALASIHSQLQGLEREAIPQFSAAQKPLLSL EETLNVTERSFHQLVALLHCRSLHKDYGSALRGLCEDALEGLLFLMLFSLLSAGALATTLCSLPRAWALF PPSDDYDDTDDDDPFNPQESKRFVQWQSSI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046310
ORF Size	1350 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046310, AAH46310
RefSeq Size	2092 bp
RefSeq ORF	1352 bp
Locus ID	57776
Gene Summary	This gene encodes a member of the Tweety family of membrane proteins. Members of this family contain five predicted transmembrane regions that are arranged in a characteristic pattern. In mouse, the protein is predominantly localized to the endoplasmic reticulum and displays calcium binding activity. Targeted knock out of this gene results in early embryonic lethality prior to the blastocyst stage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

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