Cblc (BC111037) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2310076I21Rik; 2310079L19Rik; Cbl3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207210 representing BC111037 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCGGCAGCCCCGCGAGGGTGGCAGCGGGGCGAGCCACGCGCGCTTAGCCGGGCGGTGAAGT TGCTCCAGCGCCTAGAAGAGCAATGCAGGGATCCCAGGATGGTCACGGGGCCCCCGTCCCTGCGGGACCT GCTGCCCCGCACCGCGCAGCTACTTGGAGAGGTGGCAAAGGCCCGGCGCGAGGCCAGGGAAGACCCCGAG GGTCCCGGTGGCGCCGATGACTTTCTGGCCATCTACCTGGCCAATCTGGAGGTCAAAGGCAGGCAGGTGG CGGAGCTCCTGCCACCCCGAGGCAAAAAGGACGTGAACCAGGATGTTTTCCGGGAGGGCTCCAGATTCAG GCGACAACTGGCCAAGCTGGCCCTCATCTTCAGTCACATGCACGCGGAGTTGAGCGCACTCTTCCCTGCT GGGAAGTACTGTGGGCACCTGTACCAGCTCACCAAGGGCTCTGCCCACATCTTCTGGAGGCAGAATTGTG GAGTTCGGTGCGTGCTTCCCTGGGCTGAGTTCCAGTCCCTCCTGTGTGCCTGCCACCCTGTGGAACCAGG CCCCACCATGCAGGCCTTGCGGTCCACCTTGGACCTCACCTGTAACGGCCACGTGTCTGTCTTTGAGTTT GACGTCTTCACCAGGCTCTTTCAGCCGTGGCCCACTTTACTGAGGAATTGGCAACTCCTGGCTGTCAACC ATCCTGGCTACATGGCCTTCCTCACCTACGATGAGGTCCAAACACGCCTGCAGGCCTACAGGGACAAACC AGGCAGCTATATCTTCCGGCCAAGCTGTACCCGCCTGGGGCAGTGGGCCATTGGATACGTGAGCTCCGAT GGAAGCATCCTGCAAACCATTCCTCTCAACAAACCTCTGCTGCAGGTGCTCCTGAAGGGACAAAAGGACG GCATCTTCCTCTTCCCTGATGGAAAGAAACACAACCCAGACCTGACTGAGCTCTGCCGGGTAGAACCCTA CCAACGCATCCAAGTGTCAGAGGACTCAGACAGCCAGACCTGTCCCTTCTGCCGTTGTGAGATCAAAGGT CGAGAGGCCGTGAGTATCTGTCAGGCACAGGAGAGGCCAACGGAGGTCAGGACTGCTGCAGATGACTCAA GAGACAACTGTCACCAGGAGGCTGCTGAGCAGAAACTGGGGCCGGTGATTCCCTCTGCTCCCTCACTACT CCCTGAAGATCAGTTCCCTCAGGGACCCCAAGACAAAGGCTGGCTGACACTGGCACCACTCGCCCTGCCC AGGCTCCGACCTCCACTTCCTCTCCCCAAAATGGCCTCGGTTCTGTGGGAAGTCACCTCCAGGCCCCGGG CCAGGGAGGAGGCCACAGAAAGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207210 representing BC111037 Red=Cloning site Green=Tags(s) MAAAAAPRGWQRGEPRALSRAVKLLQRLEEQCRDPRMVTGPPSLRDLLPRTAQLLGEVAKARREAREDPE GPGGADDFLAIYLANLEVKGRQVAELLPPRGKKDVNQDVFREGSRFRRQLAKLALIFSHMHAELSALFPA GKYCGHLYQLTKGSAHIFWRQNCGVRCVLPWAEFQSLLCACHPVEPGPTMQALRSTLDLTCNGHVSVFEF DVFTRLFQPWPTLLRNWQLLAVNHPGYMAFLTYDEVQTRLQAYRDKPGSYIFRPSCTRLGQWAIGYVSSD GSILQTIPLNKPLLQVLLKGQKDGIFLFPDGKKHNPDLTELCRVEPYQRIQVSEDSDSQTCPFCRCEIKG REAVSICQAQERPTEVRTAADDSRDNCHQEAAEQKLGPVIPSAPSLLPEDQFPQGPQDKGWLTLAPLALP RLRPPLPLPKMASVLWEVTSRPRAREEATESS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC111037
ORF Size	1358 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC111037, AAI11038
RefSeq Size	1527 bp
RefSeq ORF	1358 bp
Locus ID	80794
Gene Summary	Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-424', has the highest ubiquitin ligase activity among CBL family proteins. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.[UniProtKB/Swiss-Prot Function]

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