Krt15 (BC057934) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI528832; K15; Krt1-15
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207279 representing BC057934 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCACATTCTTGCAAACGTCTTCCACTTTTGGAGGCAGCTCTACCCGAGGGGCTTCCCTCCGTG CTGGGGGAGGCAGCTTTGGTGGAGGGAGTCTCTATGGGGGAGGTGGAAGCCGAAGTATCTCTGCTTCTTC TGCTAGGTTTGTCTCCTCGGGGGCAGGAGGGGGCTTTGGGGGTGGCATGAGTTGTGGCTTTGGTGGTGGT TTTGGTGGAGGCTTTGGTGGGGGTTTTGGCGGTGGCTTCGGTGATTTTGGTGGTGGCGATGGAGGTCTCC TCTCTGGCAATGAGAAGGTGACCATGCAGAACCTTAATGACCGCCTGGCCTCCTACCTGGACAAGGTGCG TGCCCTAGAGCAGGCCAACACTGAGCTGGAGGTGAAGATCCGAGATTGGTACCAGAAGCAGAGCCCAGCC AGTCCAGATCGGGACTACAGCCATTACTTTAAGACCATGGAAGAGATCCGGGACAAAATTCTGGCTGCCA CCATTGACAACTCGCGCGTTGTCCTGGAGATTGACAATGCCAGGCTGGCGGCGGACGACTTCAGGCTCAA GTATGAGAATGAGCTGACCCTGCGTCAGGGCGTGGAGGCCGACATCAACGGGCTGCGCCGGGTGCTGGAT GAGCTGACCCTGGCCAGGACCGACCTGGAGATGCAGATTGAGCAGCTGAACGAGGAGCTGGCTTACCTGA AGAAGAACCATGAGGAGGAGATGAAGGAATTCAGCAGTCAACTGGCTGGCCAGGTCAATGTGGAAATGGA CGCAGCACCCGGGGTGGACCTGACCCGCATGCTGGCAGAGATGAGGGAGCAGTATGAAGCTATTGCAGAG AAAAACCGTCGGGATGTAGAGGCCTGGTTCTTCAGTAAGACTGAAGAGCTGAACAAGGAGGTGGCGTCTA ACACAGAAATGATCCAGACCAGCAAGACGGAGATCACAGACCTGAGACGGACCCTGCAGGGGCTAGAGAT TGAGTTGCAGTCTCAGCTCAGCATGAAAGCCGGGCTGGAGAACTCTCTGGCAGAGGTGGAGTGCCGCTAT GCCACACAGCTGCAGCAGATCCAGGGGGTCATTACCGGTCTGGAGACTCAGCTGAGTGAGCTCCGCTGTG AGATGGAGGCTCAGAACCAGGAGTACAACATGCTGCTGGACATCAAGACTCGGCTGGAACAGGAGATCGC CACTTACCGGAACCTGCTCGAGGGCCAGGATGCTAAGATGGCTGGTATTGGTGTCAGAGAAGTATCCCTG GGAGGAAGCAGCGGTGGCGGTGGCAGCAGTAGCAGCAGCAGCAATTTCCATATCAGTGTGGAGGAATCAG TGGATGGAAAAGTGGTTTCTTCCCGTAAGAGAGAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207279 representing BC057934 Red=Cloning site Green=Tags(s) MATTFLQTSSTFGGSSTRGASLRAGGGSFGGGSLYGGGGSRSISASSARFVSSGAGGGFGGGMSCGFGGG FGGGFGGGFGGGFGDFGGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEQANTELEVKIRDWYQKQSPA SPDRDYSHYFKTMEEIRDKILAATIDNSRVVLEIDNARLAADDFRLKYENELTLRQGVEADINGLRRVLD ELTLARTDLEMQIEQLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRMLAEMREQYEAIAE KNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTLQGLEIELQSQLSMKAGLENSLAEVECRY ATQLQQIQGVITGLETQLSELRCEMEAQNQEYNMLLDIKTRLEQEIATYRNLLEGQDAKMAGIGVREVSL GGSSGGGGSSSSSSNFHISVEESVDGKVVSSRKREI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057934
ORF Size	1370 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057934, AAH57934
RefSeq Size	1706 bp
RefSeq ORF	1370 bp
Locus ID	16665
Gene Summary	In the absence of KRT14, makes a bona fide, but ultrastructurally distinct keratin filament network with KRT5.[UniProtKB/Swiss-Prot Function]

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