Atg4c (BC058981) Mouse Tagged ORF Clone

CAT#: MG207307

  • TrueORF®

Atg4c (tGFP-tagged) - Mouse autophagy-related 4C (yeast) (cDNA clone MGC:67363 IMAGE:6827140)



  "BC058981" in other vectors (1)

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Mouse Protein / Lysate Request

CNY 4,470.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Apg4-C, Atg4cl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207307 representing BC058981
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCTTCAGGAACAGATGAAGTTGACAAGCTAAAAACTAAATTTATATCTGCTTGGAACAATATGA
AATATAGTTGGGTGTTGAAAACAAAGACATATTTTAGTAGAAATTCACCTGTTTTATTGCTTGGAAAATG
TTATCATTTTAAATATGAAGATGAAAGCAAGATGTTGCCTGCAAGATCAGGATGTGCCATTGAAGATCAT
GTAATTGCAGGGAATGTGGAAGAGTTCCGTAAGGATTTCATTTCTAGATTATGGCTGACCTACAGGGAAG
AATTTCCTCAAATCGAAGCTTCAGCCTTGACGACAGACTGTGGCTGGGGCTGCACGCTGCGGACCGGCCA
GATGCTGCTGGCTCAGGGACTCATACTCCATTTTCTCGGTAGAGCTTGGACCTGGCCTGATGCGCTGCAC
ATTGAGAACGCAGACTCTGACTCATGGACTTCCAACACTGTCAAAAAGTTTACTGCATCGTTTGAAGCTT
CACTTTCGGGGGACAGAGAACTCAGAACTCCAGCAGTTTCTCTGAAGGAAACATCTGGGAAGTGTCCCGA
TGACCACGCAGTGCGGAATGAGGCTTATCACAGGAAGATCATCTCTTGGTTTGGAGATTCCCCCGTGGCC
GTCTTTGGCTTACATCGTCTGATAGAGTTTGGGAAGAAGTCTGGGAAAAAAGCTGGCGATTGGTATGGAC
CAGCGGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCCGGATTTGCAAGGGCTAAC
TATTTATGTTGCCCAGGACTGTACAGTTTACAATTCTGACGTGATTGATAAGCAGACTGACTCCGTGACA
GCTGGAGATGCACGTGACAAGGCTGTCATTATTTTAGTTCCTGTTAGACTCGGTGGAGAAAGGACCAATA
CCGACTATCTGGAGTTTGTGAAGGGTGTTTTAAGCCTTGAATACTGTGTAGGTATCATTGGCGGCAAACC
TAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATGGATCCTCATTACTGCCAG
TCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTCGAGACATTCCACTGTCCATCTCCTAAAAAGATGT
CATTTCGAAAAATGGATCCTAGTTGTACGATAGGATTTTACTGTCGAAATGTCCAGGACTTTGAACGAGC
GTCTGAAGAAATCACTAAGATGTTGAAAATTTCCTCTAAGGAGAAATATCCACTATTTACTTTTGTAAAT
GGCCATTCCAAAGACTTTGATTTTACATCTACCGCAGCCAGTGAAGAAGACCTTTTCTCAGAAGATGAAA
GGAAGAATTTCAAAAGATTTAGCACAGAAGAGTTTGTCCTCCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207307 representing BC058981
Red=Cloning site Green=Tags(s)

MEASGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDESKMLPARSGCAIEDH
VIAGNVEEFRKDFISRLWLTYREEFPQIEASALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALH
IENADSDSWTSNTVKKFTASFEASLSGDRELRTPAVSLKETSGKCPDDHAVRNEAYHRKIISWFGDSPVA
VFGLHRLIEFGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGLTIYVAQDCTVYNSDVIDKQTDSVT
AGDARDKAVIILVPVRLGGERTNTDYLEFVKGVLSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQ
SFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFERASEEITKMLKISSKEKYPLFTFVN
GHSKDFDFTSTAASEEDLFSEDERKNFKRFSTEEFVLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058981
ORF Size 1376 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058981, AAH58981
RefSeq Size 3232 bp
RefSeq ORF 1376 bp
Locus ID 242557
Gene Summary Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity). Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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